Effect of Bacillus subtilis PB6, a natural probiotic on colon mucosal inflammation and plasma cytokines levels in inflammatory bowel disease.

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dc.contributor.authorSelvam, Ren_US
dc.contributor.authorMaheswari, Pen_US
dc.contributor.authorKavitha, Pen_US
dc.contributor.authorRavichandran, Men_US
dc.contributor.authorSas, Benedikten_US
dc.contributor.authorRamchand, C Nen_US
dc.date.accessioned2009-02-21en_US
dc.date.accessioned2009-05-27T09:50:41Z
dc.date.available2009-02-21en_US
dc.date.available2009-05-27T09:50:41Z
dc.date.issued2009-02-21en_US
dc.description.abstractThe pathophysiology of inflammatory bowel disease (IBD) involves the production of diverse lipid mediators, namely eicosanoid, lysophospholipids, and platelet-activating factor, in which phospholipase A2 (PLA2) is the key enzyme. Thus, it has been postulated that control of lipid mediators production by inhibition of PLA2 would be useful for the treatment of IBD. This hypothesis has been tested in the present study by examining the therapeutic effect of a novel natural probitic Bacillus subtilis PB6 (ATCC- PTA 6737). B. subtilis PB6 is found to secrete surfactins (cyclic lipopeptides) which have anti-bacterial potential. These surfactins inhibit PLA2, a rate-limiting enzyme involved in the arachidonic acid associated inflammatory pathway and could downregulate the inflammatory response by regulating the eicosanoid and cytokine pathways. With this concept, an experimental animal trial has been conducted in a rat model of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. The oral administration of PB6 suppresses the colitis as measured by mortality rate, changes in the weight gain, colon morphology and the levels of plasma cytokines. The animals treated orally with PB6 at 1.5 x 10(8) CFU/kg thrice daily from day 4 to 10 significantly improve gross pathology of the colon and regain the colon weight to normal (p < 0.05), compared to TNBS-induced positive control. The plasma levels of pro-inflammatory cytokines (TNF-alpha, 1L-1beta, IL-6 and IFN-gamma) are also significantly lowered (p < 0.05) and anti-inflammatory cytokine (IL-I0 and TGF-beta) significantly (p < 0.05) increased after the oral administration of PB6 on day 11. The present study supports the concept that PB6 inhibits PLA2 by the secreting surfactins. In a clinical investigation, it is found to be well tolerated by all the healthy volunteers.en_US
dc.description.affiliationKemin Industries South Asia (P) Ltd, Plot # K3, 11th Cross Street, SIPCOT Industrial complex, Gummidipoondi, 601 201, Tamil Nadu, India.en_US
dc.identifier.citationSelvam R, Maheswari P, Kavitha P, Ravichandran M, Sas B, Ramchand CN. Effect of Bacillus subtilis PB6, a natural probiotic on colon mucosal inflammation and plasma cytokines levels in inflammatory bowel disease. Indian Journal of Biochemistry & Biophysics. 2009 Feb; 46(1): 79-85en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28730
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimals
dc.subject.meshBacillus subtilis
dc.subject.meshBacterial Proteins --metabolism
dc.subject.meshBody Weight
dc.subject.meshColitis, Ulcerative --blood
dc.subject.meshColitis, Ulcerative --chemically induced
dc.subject.meshColitis, Ulcerativem --microbiology
dc.subject.meshColitis, Ulcerative --therapy
dc.subject.meshColon --immunology
dc.subject.meshColon --microbiology
dc.subject.meshColon --pathology
dc.subject.meshCytokines --blood
dc.subject.meshDisease Models, Animal
dc.subject.meshIntestinal Mucosa --immunology
dc.subject.meshIntestinal Mucosa --microbiology
dc.subject.meshIntestinal Mucosa --pathology
dc.subject.meshLipopeptides --metabolism
dc.subject.meshMale
dc.subject.meshOrgan Size
dc.subject.meshPeptides, Cyclic --metabolism
dc.subject.meshPhospholipases A2 --metabolism
dc.subject.meshProbiotics
dc.subject.meshRandom Allocation
dc.subject.meshRats
dc.subject.meshRats, Wistar
dc.subject.meshTrinitrobenzenesulfonic Acid
dc.titleEffect of Bacillus subtilis PB6, a natural probiotic on colon mucosal inflammation and plasma cytokines levels in inflammatory bowel disease.en_US
dc.typeJournal Articleen_US
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