Clinico-microbiological Profile of Urinary Tract Infection with Special Reference to Uropathogenic E coli : Antibiotic susceptibility Pattern, Phylogenetic Background and Virulent Factor Distribution from West Bengal, India

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dc.contributor.authorKoley, Snehashisen_US
dc.contributor.authorMukherjee, Mandiraen_US
dc.contributor.authorHalder, Prantikien_US
dc.contributor.authorBose, Ambaren_US
dc.contributor.authorLahre, Dushyanten_US
dc.contributor.authorMukhopadhyay, Sumien_US
dc.contributor.authorMallik, Sudeshnaen_US
dc.date.accessioned2023-06-17T08:35:26Z
dc.date.available2023-06-17T08:35:26Z
dc.date.issued2022-10
dc.description.abstractBackground and Objectives : Routine surveillance and monitoring studies pose a constant need to update clinicians on prevalent pathogens and rational and empirical treatment in Urinary Tract Infection (UTI). Escherichia coli (E coli) is the most commonly isolated uropathogen globally. Extended-Spectrum ?-Lactamase (ESBL) production and ?-Lactamase Inhibitor Resistance (BLIR) among these pathogens together with their uro-virulence determinants further complicate treatment approaches. This study investigated the clinico-microbiological pattern of UTI and determined the antibiotic sensitivity pattern, the phylogenetic background, and virulence determinants of E coli, the most commonly isolated uropathogen. Methods : Uropathogens isolated by urine culture from community and hospitalized patients were biochemically speciated. Antibiotic susceptibility was tested by Kirby-bauer disk diffusion method. Phylogenetic background and virulence determinants of E coli isolates were identified by PCR. SPSS 16.0 was used for statistical interpretation. Results : 45% of the urine samples showed growth positivity. 44% amongst them were E coli. All isolates were multidrug-resistant. 50% and 40% were ESBL producers and BLIR respectively. Former showed highest resistance to quinolone, fluoroquinolones, cotrimoxazole, and latter were resistant against all drugs tested except nitrofurantoin. Significant correlation existed between the ?-lactams, quinolone, fluoroquinolones, cotrimoxazole (p<0.05) resistance pattern. BLIR and ESBL E coli recorded highest prevalence of pathogenic phylogroup B2 and D respectively. Varied prevalence of fimbrial (fimH, papC, papEF, papG, GII) and toxin genes (iroN, hlyA, cnfI, i ucD, cdtBU) in ESBL, BLIR and non-ESBL isolates were observed. Their distribution was statistically significant (p=0.05). Interpretation and Conclusions : Nitrofurantoin is the drug of choice in empirical treatment of uncomplicated UTI. Aggressive and consistent investigation and health education are highly recommended for effective clinical management in UTI.en_US
dc.identifier.affiliationsDepartment of Biochemistry and Medical Biotechnology, School of Tropical Medicine, Kolkata 700073en_US
dc.identifier.affiliationsM Tech, Research Scholaren_US
dc.identifier.affiliationsPhD, Associate Professoren_US
dc.identifier.affiliationsMD, Assistant Professor, Department of Tropical Medicineen_US
dc.identifier.affiliationsMSc, Research Scholaren_US
dc.identifier.affiliationsMD, Senior Resident, Department of Tropical Medicineen_US
dc.identifier.affiliationsPhD, Research Associate, Department of Laboratory Medicineen_US
dc.identifier.affiliationsMD, Associate Professor, Department of Tropical Medicine and Correspondence Authoren_US
dc.identifier.citationKoley Snehashis, Mukherjee Mandira, Halder Prantiki, Bose Ambar, Lahre Dushyant, Mukhopadhyay Sumi, Mallik Sudeshna. Clinico-microbiological Profile of Urinary Tract Infection with Special Reference to Uropathogenic E coli : Antibiotic susceptibility Pattern, Phylogenetic Background and Virulent Factor Distribution from West Bengal, India. Journal of The Indian Medical Association. 2022 Oct; 120(10): 48-53en_US
dc.identifier.issn0019-5847
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/216630
dc.languageenen_US
dc.publisherIndian Medical Associationen_US
dc.relation.issuenumber10en_US
dc.relation.volume120en_US
dc.source.urihttps://www.onlinejima.com/read_journals.php?article=1035en_US
dc.subjectAntibiotic resistanceen_US
dc.subjectPhylogenetic backgrounden_US
dc.subjectVirulence factoren_US
dc.subjectUropathogenic E colien_US
dc.subjectUrinary Tract Infectionen_US
dc.titleClinico-microbiological Profile of Urinary Tract Infection with Special Reference to Uropathogenic E coli : Antibiotic susceptibility Pattern, Phylogenetic Background and Virulent Factor Distribution from West Bengal, Indiaen_US
dc.typeJournal Articleen_US
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