A novel analytical liquid chromatography–tandem mass spectrometry method for the estimation of Ribavirin in bulk and pharmaceutical formulation

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dc.contributor.authorSharma, Prachien_US
dc.contributor.authorS.T, Narenderanen_US
dc.contributor.authorS.N, Meyyanathanen_US
dc.contributor.authorSangamithra, Ren_US
dc.contributor.authorMohire, Sourabh Sanjayen_US
dc.contributor.authorBabu, Ben_US
dc.contributor.authorKalaivani, Men_US
dc.date.accessioned2020-10-16T08:56:38Z
dc.date.available2020-10-16T08:56:38Z
dc.date.issued2020-01
dc.description.abstractThe aim of this study is to develop and validate a method that is simple, precise, sensitive, and rapid compared tousing the liquid chromatography–tandem mass spectrometry method for the quantitative determination of Ribavirin(antiviral drug) in its tablet formulation. The development and validation of the method were achieved using a column(Zorbax 50 mm × 4.6 mm × 5 µm) with mobile phase ammonium formate (pH: 7.50): acetonitrile in the ratio (30:70,v/v) with the flow rate of 0.5 ml/min. The retention time for Ribavirin was 1.1 minutes with the total run time of2.5 minutes. The linearity range for Ribavirin was from 2 to 100 ng/ml with a correlation coefficient of 0.9956. Thedetection and quantitation limits of Ribavirin are 0.7 and 2 ng/ml, respectively. The percentage recovery of Ribavirinranged from 94.00% to 98.33%. The percentage relative standard deviation for intraday and interday precision resultswas found to be 0.67%–2.11% and 1.92%–3.11%, respectively. The new method developed for Ribavirin drug wasfound to be rapid, sensitive, selective, and economical. The established method was the evaluation of Ribavirin in itsmarketed formulation (tablet). The values obtained from the analysis were found out to be within the acceptable limitsas per the International Council for Harmonisation (ICH) guidelines.en_US
dc.identifier.affiliationsDepartment of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Pharmaceutical Analysis, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsIndian Pharmacopoeia Commission, New Delhi, India.en_US
dc.identifier.citationSharma Prachi, S.T Narenderan, S.N Meyyanathan, Sangamithra R, Mohire Sourabh Sanjay, Babu B, Kalaivani M. A novel analytical liquid chromatography–tandem mass spectrometry method for the estimation of Ribavirin in bulk and pharmaceutical formulation. Journal of Applied Pharmaceutical Science. 2020 Jan; 2020 Jan: 096-100en_US
dc.identifier.issn2231-3354
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/210635
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber1en_US
dc.relation.volume10en_US
dc.source.urihttps://dx.doi.org//10.7324/JAPS.2020.101013en_US
dc.subjectRibavirinen_US
dc.subjectformulationen_US
dc.subjectLC-MS/MSen_US
dc.subjectvalidationen_US
dc.subjectICHen_US
dc.titleA novel analytical liquid chromatography–tandem mass spectrometry method for the estimation of Ribavirin in bulk and pharmaceutical formulationen_US
dc.typeJournal Articleen_US
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