An aminopeptidase regulates LPS stimulated interleukin-8 receptor on the surface of human neutrophils.

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dc.contributor.authorBhattacharya, Cen_US
dc.contributor.authorManna, S Ken_US
dc.contributor.authorSamanta, Sen_US
dc.contributor.authorGupta, S Ken_US
dc.contributor.authorSamanta, A Ken_US
dc.date.accessioned1997-02-01en_US
dc.date.accessioned2009-05-27T09:28:26Z
dc.date.available1997-02-01en_US
dc.date.available2009-05-27T09:28:26Z
dc.date.issued1997-02-01en_US
dc.description.abstractA large number of inflammatory diseases are mediated by interleukin-8, an inflammatory neutrophil chemotactic agent. Since the cytokine acts through a cell surface receptor, detailed knowledge about the regulation of receptor expression is very important. We found that LPS in serum became activated and triggered the expression of IL-8 receptor by more than two folds within 30 min. After that period, the receptor attained normal level within 2 hr of SA-LPS stimulation. EDTA and bestatin could block this downregulation of IL-8 receptor. Intracellular Ca2+ level was increased till 45 min of SA-LPS stimulation and then the level was reduced. Addition of CaCl2 accelerated and depletion of Ca2+ inhibited the downregulation of the IL-8 receptor. The ligand could fully protect the loss of receptor from downregulation. It suggests that during SA-LPS stimulation, increase in intracellular Ca2+ level activates an aminopeptidase which presumably cleaves the N-terminal region of the receptor, critically essential for the function of IL-8. Thus the activated aminopeptidase regulates the functions of IL-8. The study is important for understanding the regulation of IL-8 receptor expression by LPS during bacterial infection.en_US
dc.description.affiliationDivision of Biometry, Indian Statistical Institute, Calcutta, India.en_US
dc.identifier.citationBhattacharya C, Manna SK, Samanta S, Gupta SK, Samanta AK. An aminopeptidase regulates LPS stimulated interleukin-8 receptor on the surface of human neutrophils. Indian Journal of Biochemistry & Biophysics. 1997 Feb-Apr; 34(1-2): 214-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27613
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAminopeptidases --metabolismen_US
dc.subject.meshAntigens, CD --metabolismen_US
dc.subject.meshBacterial Infections --immunologyen_US
dc.subject.meshCalcium --metabolismen_US
dc.subject.meshCell Membrane --immunologyen_US
dc.subject.meshEnzyme Activationen_US
dc.subject.meshHumansen_US
dc.subject.meshLipopolysaccharides --pharmacologyen_US
dc.subject.meshNeutrophils --immunologyen_US
dc.subject.meshReceptors, Interleukin --metabolismen_US
dc.subject.meshReceptors, Interleukin-8Aen_US
dc.titleAn aminopeptidase regulates LPS stimulated interleukin-8 receptor on the surface of human neutrophils.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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