Isolation and PCR detection of rickettsiae from clinical and rodent samples in Malaysia.

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dc.contributor.authorTay, S Ten_US
dc.contributor.authorRohani, M Yen_US
dc.contributor.authorHo, T Men_US
dc.contributor.authorDevi, Sen_US
dc.date.accessioned2009-05-27T16:06:01Z
dc.date.available2009-05-27T16:06:01Z
dc.date.issued2002-12-22en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractIsolation of rickettsiae from patients' blood samples and organ samples of wild rodents from areas with high seroprevalence of rickettsial infections was attempted using cell culture assay and animal passages. L929 mouse fibroblast cells grown in 24 well tissue culture plate were inoculated with buffy coat of febrile patients and examined for the growth of rickettsiae by Giemsa, Gimenez staining and direct immunofluorescence assay. No rickettsiae were isolated from 48 patients' blood samples. No symptomatic infections were noted in mice or guinea pigs infected with 50 organ samples of wild rodents. There was no rickettsial DNA amplified from these samples using various PCR detection systems for Orientia tsutsugamushi, typhus and spotted fever group rickettsiae.en_US
dc.description.affiliationDepartment of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia. tayst@ummc.edu.myen_US
dc.identifier.citationTay ST, Rohani MY, Ho TM, Devi S. Isolation and PCR detection of rickettsiae from clinical and rodent samples in Malaysia. The Southeast Asian Journal of Tropical Medicine and Public Health. 2002 Dec; 33(4): 772-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34415
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2002_33_4/19-2877.pdfen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial --blooden_US
dc.subject.meshAzure Stainsen_US
dc.subject.meshCell Culture Techniquesen_US
dc.subject.meshChilden_US
dc.subject.meshDNA, Bacterial --analysisen_US
dc.subject.meshFemaleen_US
dc.subject.meshFluorescent Antibody Technique, Directen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshMalaysia --epidemiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshPopulation Surveillanceen_US
dc.subject.meshRickettsia --classificationen_US
dc.subject.meshRickettsia Infections --blooden_US
dc.subject.meshRodent Diseases --blooden_US
dc.subject.meshRodentiaen_US
dc.subject.meshSeroepidemiologic Studiesen_US
dc.titleIsolation and PCR detection of rickettsiae from clinical and rodent samples in Malaysia.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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