Hepatitis G virus infection in India: prevalence and phylogenetic analysis based on 5' non-coding region.

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dc.contributor.authorArankalle, V Aen_US
dc.contributor.authorDeshmukh, T Men_US
dc.contributor.authorChobe, L Pen_US
dc.contributor.authorChadha, M Sen_US
dc.contributor.authorWalimbe, A Men_US
dc.date.accessioned2001-01-24en_US
dc.date.accessioned2009-05-29T02:24:04Z
dc.date.available2001-01-24en_US
dc.date.available2009-05-29T02:24:04Z
dc.date.issued2001-01-24en_US
dc.description.abstractOBJECTIVES: To determine the prevalence of hepatitis G virus (HGV) infection in western India and to carry out phylogenetic analysis of HGV isolates. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect HGV RNA in serum samples obtained from paid plasma donors, patients with hemophilia and voluntary blood donors. Nine Indian and one Kenyan HGV RNA-positive samples were sequenced in the 5' non-coding region (5'-NCR). Phylogenetic analysis based on the comparison of a 101 nucleotide fragment from a large number of HGV isolates from 22 countries (including Indian and Kenyan sequences obtained during the present study) was carried out. RESULTS: HGV RNA positivity rates among paid plasma donors from a commercial plasmapheresis unit (7/43, 16.3%) and patients with hemophilia (5/44, 11.4%) were significantly higher than that in voluntary blood donors (0/51; p=0.003 and 0.019, respectively). Among patients with acute non-A to E hepatitis and fulminant hepatic failure, 1 of 50 and 1 of 28 were HGV RNA-positive, whereas 6 of 49 (12%) patients with chronic liver disease had circulating HGV RNA. All Indian isolates belonged to genotype 2, whereas the Kenyan isolate formed a distinct branch within genotype 1 consisting of African isolates. CONCLUSION: Our results suggest existence of parenteral transmission of HGV in the Indian population. HGV was not an important cause of acute non-A to E hepatitis or fulminant hepatic failure among the patients investigated. Genotype 2 seems to be the most prevalent genotype in western India.en_US
dc.description.affiliationHepatitis Division, National Institute of Virology, Pune. aarankalle@hotmail.comen_US
dc.identifier.citationArankalle VA, Deshmukh TM, Chobe LP, Chadha MS, Walimbe AM. Hepatitis G virus infection in India: prevalence and phylogenetic analysis based on 5' non-coding region. Indian Journal of Gastroenterology. 2001 Jan-Feb; 20(1): 13-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/64096
dc.language.isoengen_US
dc.source.urihttps://www.indianjgastro.comen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlaviviridae --geneticsen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHepatitis, Viral, Human --diagnosisen_US
dc.subject.meshHumansen_US
dc.subject.meshIndia --epidemiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPolymorphism, Single Nucleotideen_US
dc.subject.meshPrevalenceen_US
dc.subject.meshRNA, Viral --analysisen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshRisk Factorsen_US
dc.subject.meshSeroepidemiologic Studiesen_US
dc.titleHepatitis G virus infection in India: prevalence and phylogenetic analysis based on 5' non-coding region.en_US
dc.typeJournal Articleen_US
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