Isolation of tubercle bacilli from sputum samples of patients in the field studies by the cetylpyridinium chloride-sodium chloride & sodium hydroxide methods.

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dc.contributor.authorSelvakumar, Nen_US
dc.contributor.authorVanajakumar,en_US
dc.contributor.authorGopi, P Gen_US
dc.contributor.authorVenkataramu, K Ven_US
dc.contributor.authorDatta, Men_US
dc.contributor.authorParamasivan, C Nen_US
dc.contributor.authorPrabhakar, Ren_US
dc.date.accessioned1995-10-01en_US
dc.date.accessioned2009-05-27T08:40:31Z
dc.date.available1995-10-01en_US
dc.date.available2009-05-27T08:40:31Z
dc.date.issued1995-10-01en_US
dc.description.abstractA total of 125 sputum specimens, collected in the field, were homogenised, aliquoted in sterile universal containers and randomly allocated to the cetylpyridinium chloride - sodium chloride (CPC-NaCl) method and sodium hydroxide (NaOH) method for culture of tubercle bacilli. After storage for 8 days at ambient temperature in the field laboratory at Thiruvallur, the aliquots were transported to the main laboratory at Madras where they were processed for culture by the respective methods. The yield of positive cultures in the CPC-NaCl (31/125) method was only marginally better than that in the NaOH method (27/125) (95% CI being-3.4 to 9.8%), while the contamination of cultures was significantly less in the CPC-NaCl method (3/125) than in the NaOH method (12/125) (95% CI being 2.2 to 12.2%). As the CPC-NaCl method has advantages over the NaOH method in reducing contamination, in augmenting the yield of positive cultures and also in its simplicity, it can be applied in field studies.en_US
dc.description.affiliationTuberculosis Research Centre, Madras.en_US
dc.identifier.citationSelvakumar N, Vanajakumar , Gopi PG, Venkataramu KV, Datta M, Paramasivan CN, Prabhakar R. Isolation of tubercle bacilli from sputum samples of patients in the field studies by the cetylpyridinium chloride-sodium chloride & sodium hydroxide methods. Indian Journal of Medical Research. 1995 Oct; 102(): 149-51en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/25261
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshBacteriological Techniquesen_US
dc.subject.meshCetylpyridiniumen_US
dc.subject.meshHumansen_US
dc.subject.meshMycobacteria, Atypical --isolation & purificationen_US
dc.subject.meshSodium Chlorideen_US
dc.subject.meshSodium Hydroxideen_US
dc.subject.meshSpecimen Handling --methodsen_US
dc.subject.meshSputum --microbiologyen_US
dc.titleIsolation of tubercle bacilli from sputum samples of patients in the field studies by the cetylpyridinium chloride-sodium chloride & sodium hydroxide methods.en_US
dc.typeClinical Trialen_US
dc.typeJournal Articleen_US
dc.typeRandomized Controlled Trialen_US
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