Similar regeneration of articular cartilage defects with autologous & allogenic chondrocytes in a rabbit model

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dc.contributor.authorBoopalan, P Ren_US
dc.contributor.authorVarghese, VDen_US
dc.contributor.authorSathishkumar, Sen_US
dc.contributor.authorArumugam, Sen_US
dc.contributor.authorAmarnath, Ven_US
dc.date.accessioned2020-04-10T01:42:37Z
dc.date.available2020-04-10T01:42:37Z
dc.date.issued2019-05
dc.description.abstractBackground & objectives: Articular cartilage defects in the knee have a very poor capacity for repair due to avascularity. Autologous chondrocyte transplantation (ACT) is an established treatment for articular cartilage defects. Animal studies have shown promising results with allogenic chondrocyte transplantation since articular cartilage is non-immunogenic. In addition to being economical, allogenic transplantation has less morbidity compared to ACT. This study was undertaken to compare ACT with allogenic chondrocyte transplantation in the treatment of experimentally created articular cartilage defects in rabbit knee joints. Methods: Cartilage was harvested from the left knee joints of six New Zealand white rabbits (R1-R6). The harvested chondrocytes were cultured to confluence and transplanted onto a 3.5 mm chondral defect in the right knees of 12 rabbits [autologous in 6 rabbits (R1-R6) and allogenic in 6 rabbits (R7-R12)]. After 12 wk, the rabbits were euthanized and histological evaluation of the right knee joints were done with hematoxylin and eosin and safranin O staining. Quality of the repair tissue was assessed by the modified Wakitani histological grading scale. Results: Both autologous and allogenic chondrocyte transplantation resulted in the regeneration of hyaline/mixed hyaline cartilage. The total histological scores between the two groups showed no significant difference. Interpretation & conclusions: Allogenic chondrocyte transplantation seems to be as effective as ACT in cartilage regeneration, with the added advantages of increased cell availability and reduced morbidity of a single surgery.en_US
dc.identifier.affiliationsDepartment of Orthopaedics; Department of Center for Stem Cell Research, Christian Medical College, Vellore, Indiaen_US
dc.identifier.affiliationsDepartment of Orthopaedics, Christian Medical College, Vellore, Indiaen_US
dc.identifier.affiliationsDepartment of Physiology, Christian Medical College, Vellore, Indiaen_US
dc.identifier.affiliationsHistopathology Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Thiruvananthapuram, Indiaen_US
dc.identifier.affiliationsBone & Joint Hospital, Tiruchirappalli, Indiaen_US
dc.identifier.citationBoopalan P R, Varghese VD, Sathishkumar S, Arumugam S, Amarnath V. Similar regeneration of articular cartilage defects with autologous & allogenic chondrocytes in a rabbit model. Indian Journal of Medical Research. 2019 May; 149(5): 650-655en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195874
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber5en_US
dc.relation.volume149en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1233_17en_US
dc.subjectAutologous chondrocyte transplantationen_US
dc.subjectautologous versus allogenicen_US
dc.subjectcartilage cultureen_US
dc.subjectWakitani scoreen_US
dc.titleSimilar regeneration of articular cartilage defects with autologous & allogenic chondrocytes in a rabbit modelen_US
dc.typeJournal Articleen_US
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