Glycolipid composition of a mutant cell line of mouse FM3A cells, and the effect of exogenous glycolipids on cell growth.

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dc.contributor.authorHanda, Sen_US
dc.contributor.authorTaki, Ten_US
dc.contributor.authorOgura, Ken_US
dc.contributor.authorRokukawa, Cen_US
dc.date.accessioned1990-12-01en_US
dc.date.accessioned2009-05-27T09:32:46Z
dc.date.available1990-12-01en_US
dc.date.available2009-05-27T09:32:46Z
dc.date.issued1990-12-01en_US
dc.description.abstractHad-1 isolated from mouse mammary tumour FM3A cells as a non-permissive cell line to Newcastle disease virus infection is deficient in NDV receptors, and galactosylation of the complex type sugar chains of the glycoproteins is extensively reduced compared to FM3A cells. It is also deficient in UDP-galactose transport into Golgi vesicles. The major neutral glycolipids in FM3A is Lac-Cer, whereas, in Had-1 cell, Glc-Cer is the major glycolipid and the concentration of neutral glycolipids is one-tenth as low as that in FM3A. GM3, GD3 and sialyl i- and I-type lactosaminylceramide are the gangliosides present in both FM3A and Had-1, although their presence in both cells is only in traces. Had-1 contains relatively high N-glycolyl-neuraminic acid. Among the several glycolipids tested, Lac-Cer, Gg-4-Cer and Glc-Cer showed inhibitory effect on proliferation of Had-1 cells, but did not show any appreciable effect on that of FM3A cells. Lac-Cer had the most potent inhibitory effect and this inhibitory effect was completely reversible. While mice injected with 5 x 10(6) cells of FM3A died in one month, those injected of Had-1 cells at the same dose survived for more than 6 months. Thus glycolipids on the cell surface play an essential role during cell growth both in vivo and in vitro.en_US
dc.description.affiliationDepartment of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, Japan.en_US
dc.identifier.citationHanda S, Taki T, Ogura K, Rokukawa C. Glycolipid composition of a mutant cell line of mouse FM3A cells, and the effect of exogenous glycolipids on cell growth. Indian Journal of Biochemistry & Biophysics. 1990 Dec; 27(6): 430-2en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27831
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Division --drug effectsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshGlycolipids --chemistryen_US
dc.subject.meshMembrane Lipids --chemistryen_US
dc.subject.meshMiceen_US
dc.subject.meshMutationen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleGlycolipid composition of a mutant cell line of mouse FM3A cells, and the effect of exogenous glycolipids on cell growth.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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