A sensitive assay for anti-HIV-1 drug discovery in a biological safety level-2 laboratory.
dc.contributor.author | Yoosook, C | en_US |
dc.contributor.author | Reutrakul, V | en_US |
dc.contributor.author | Santisuk, T | en_US |
dc.contributor.author | Chaichana, S | en_US |
dc.contributor.author | Pezzuto, J M | en_US |
dc.contributor.author | Dong, Y | en_US |
dc.contributor.author | Clanton, D J | en_US |
dc.contributor.author | Kiser, R | en_US |
dc.contributor.author | Staley, P | en_US |
dc.date.accessioned | 2009-05-27T15:34:46Z | |
dc.date.available | 2009-05-27T15:34:46Z | |
dc.date.issued | 1998-12-20 | en_US |
dc.description | The Southeast Asian Journal of Tropical Medicine and Public Health. | en_US |
dc.description.abstract | Studies involving infectious, wild type HIV-1 must be performed under strict BSL-3 practice. We have employed a defective (deltaTat/Rev)MC99 and cloned 1A2 line, ie, mutated HIV-1 and Tat/Rev transfected cells to verify anti-HIV-1 activity in a BSL-2 laboratory. A number of extracts from various parts of 11 species of plants were studied. Results were correlated with those of an anti-HIV-1 reverse transcriptase (RT) assay. | en_US |
dc.description.affiliation | Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. | en_US |
dc.identifier.citation | Yoosook C, Reutrakul V, Santisuk T, Chaichana S, Pezzuto JM, Dong Y, Clanton DJ, Kiser R, Staley P. A sensitive assay for anti-HIV-1 drug discovery in a biological safety level-2 laboratory. The Southeast Asian Journal of Tropical Medicine and Public Health. 1998 Dec; 29(4): 758-62 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/33137 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.tm.mahidol.ac.th/seameo/1998-29-4/1998-29-4-758.pdf | en_US |
dc.subject.mesh | Anti-HIV Agents | en_US |
dc.subject.mesh | Biological Assay --methods | en_US |
dc.subject.mesh | Cytoprotection --drug effects | en_US |
dc.subject.mesh | Drug Design | en_US |
dc.subject.mesh | Giant Cells --drug effects | en_US |
dc.subject.mesh | HIV Reverse Transcriptase --antagonists & inhibitors | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Laboratory Infection --prevention & control | en_US |
dc.subject.mesh | Plant Extracts --therapeutic use | en_US |
dc.subject.mesh | Reverse Transcriptase Inhibitors | en_US |
dc.title | A sensitive assay for anti-HIV-1 drug discovery in a biological safety level-2 laboratory. | en_US |
dc.type | Journal Article | en_US |
dc.type | Research Support, Non-U.S. Gov't | en_US |