New cost-effective human leukocyte antigen testing algorithm for screening of human leukocyte antigen-matched related donor in thalassemia major patient pretransplant workup: A single-center study from resource-constrained settings

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dc.contributor.authorSingh, Parvinden_US
dc.contributor.authorTiwari, Aseem Kumaren_US
dc.contributor.authorMishra, Vikash Chandraen_US
dc.contributor.authorChandra, Dineshen_US
dc.contributor.authorBhardwaj, Amit Ken_US
dc.contributor.authorDey, Nikkien_US
dc.contributor.authorRaina, Vimarshen_US
dc.date.accessioned2023-08-09T06:10:29Z
dc.date.available2023-08-09T06:10:29Z
dc.date.issued2023-06
dc.description.abstractIntroduction: India has a huge disease burden of thalassemia major with an estimated 40 million carriers and over a million thalassemia major patients. Very few patients are optimally treated, and the standard of care “hematopoietic stem cell transplant” (HSCT) is out of reach for most patients and their families. The cost of HSCT is significant, and a substantial proportion of it goes to human leukocyte antigen (HLA) testing of family members (HLA screening) in hope of getting a matched related donor (MRD) for HSCT. The aim of this study was to establish that a new proposed testing algorithm of HLA typing would be more cost-effective as compared to the conventional HLA screening within MRD families for possible HSCT. Material and Methods: Buccal swab samples of 177 thalassemia patients and their prospective family donors (232) were collected. Using a new HLA testing algorithm, samples were tested for HLA typing in a sequential manner (first HLA-B, then HLA-A, and finally HLA-DR) using the sequence-specific oligonucleotide probe method on the Luminex platform. Results: The new sequential HLA-A, HLA-B, and HLA-DRB1 testing algorithm showed a 49.1% reduction in cost compared to the conventional HLA testing algorithm. Furthermore, 40 patients (22.59%) were found to have HLA-MRD within the family among other samples that were tested. Conclusion: The new HLA testing algorithm proposed in the present study for identifying MRD for HSCT resulted in a substantial reduction in the cost of HSCT workup.en_US
dc.identifier.affiliationsChimera Transplant Research Foundation, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Transfusion Medicine, Medanta?The Medicity, Gurgaon, Haryana, Indiaen_US
dc.identifier.citationSingh Parvind, Tiwari Aseem Kumar, Mishra Vikash Chandra, Chandra Dinesh, Bhardwaj Amit K, Dey Nikki, Raina Vimarsh. New cost-effective human leukocyte antigen testing algorithm for screening of human leukocyte antigen-matched related donor in thalassemia major patient pretransplant workup: A single-center study from resource-constrained settings. Indian Journal of Community & Family Medicine. 2023 Jun; 9(1): 35-40en_US
dc.identifier.issn2395-2113
dc.identifier.issn2666-3120
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/221888
dc.languageenen_US
dc.publisherWolters Kluwer-Medknow Publicationsen_US
dc.relation.issuenumber1en_US
dc.relation.volume9en_US
dc.source.urihttps://doi.org/10.4103/ijcfm.ijcfm_20_22en_US
dc.subjectBone marrow transplanten_US
dc.subjectcost-effectivenessen_US
dc.subjectHuman Leukocyte Antigen typingen_US
dc.subjectliving-related donoren_US
dc.subjectthalassemia majoren_US
dc.titleNew cost-effective human leukocyte antigen testing algorithm for screening of human leukocyte antigen-matched related donor in thalassemia major patient pretransplant workup: A single-center study from resource-constrained settingsen_US
dc.typeJournal Articleen_US
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