Differences in the levels of aminoacylation and contents of modified nucleotides between total tRNAs from N2- and NH4(+)-grown Azospirillum lipoferum cells.

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dc.contributor.authorJayabaskaran, Cen_US
dc.contributor.authorSivakamasundari, Ren_US
dc.date.accessioned1994-12-01en_US
dc.date.accessioned2009-05-27T09:58:00Z
dc.date.available1994-12-01en_US
dc.date.available2009-05-27T09:58:00Z
dc.date.issued1994-12-01en_US
dc.description.abstractTotal tRNAs isolated from N2- and NH4(+)-grown Azospirillum lipoferum cells were compared with respect to amino acid acceptance, isoacceptor tRNA species levels and extent of nucleotide modifications. Amino-acylation of these two tRNA preparations with ten different amino acids indicated differences in the relative acceptor activities. Comparison of aminoacyl-tRNA patterns by RPC-5 column chromatography revealed no qualitative differences in the elution profiles. However, quantitative differences in the relative amounts of some isoacceptors were observed. These results indicate that alterations of relative amounts of functional tRNA species occur to match cellular requirements of the bacterial cells using N2 or NH4+ as nitrogen source. In addition, the content of modified nucleotides in total tRNAs of N2- and NH4(+)-grown cells was determined. In the NH4(+)-grown cells, content of most of the modified nucleotides decreased significantly. Based upon these results, the relationship of chargeability of tRNAs to base modifications is discussed.en_US
dc.description.affiliationDepartment of Biochemistry, Indian Institute of Science, Bangalore.en_US
dc.identifier.citationJayabaskaran C, Sivakamasundari R. Differences in the levels of aminoacylation and contents of modified nucleotides between total tRNAs from N2- and NH4(+)-grown Azospirillum lipoferum cells. Indian Journal of Biochemistry & Biophysics. 1994 Dec; 31(6): 454-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/29087
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAcylationen_US
dc.subject.meshAmino Acids --metabolismen_US
dc.subject.meshAzospirillum --drug effectsen_US
dc.subject.meshNitrogen --pharmacologyen_US
dc.subject.meshNucleotides --metabolismen_US
dc.subject.meshQuaternary Ammonium Compounds --pharmacologyen_US
dc.subject.meshRNA Processing, Post-Transcriptionalen_US
dc.subject.meshRNA, Transfer --metabolismen_US
dc.titleDifferences in the levels of aminoacylation and contents of modified nucleotides between total tRNAs from N2- and NH4(+)-grown Azospirillum lipoferum cells.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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