Temephos resistance in two forms of Aedes aegypti and its significance for the resistance mechanism.

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dc.contributor.authorPaeporn, Pungasemen_US
dc.contributor.authorKomalamisra, Narumonen_US
dc.contributor.authorDeesin, Vanidaen_US
dc.contributor.authorRongsriyam, Yuphaen_US
dc.contributor.authorEshita, Yukien_US
dc.contributor.authorThongrungkiat, Supatraen_US
dc.date.accessioned2009-05-27T16:03:41Z
dc.date.available2009-05-27T16:03:41Z
dc.date.issued2003-12-30en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractAedes aegypti, at the larval stage, has been subjected to the temephos selection in laboratory. The level of temephos resistance was detected in a microplate by biochemical assay using WHO bioassay technique. The major enzyme-based resistance mechanisms involved in temephos resistance include elevated nonspecific esterase, oxidase and insensitive acetylcholinesterase. After 19 generations of temephos selection, the selected group showed resistance ratios of 4.64 and 16.92, when compared with a non-selected group and the WHO susceptible strain, respectively. The two seperated forms, type form and the pale form of Ae. aegypti showed low levels of resistance to temephos after 19 generations of selection, with resistance ratios of 4.82 and 4.07 for the type form and the pale form, respectively; when compared with the non-selected strain, 17.58 and 14.84, when compared with the WHO susceptible strain. This showed that the type form could develop higher level resistance than the pale form. The esterase inhibitor (S,S,S-tributyl phosphorotrithioate, DEF) or synergist implicated detoxifying esterase in all the temephos selected groups and the presence of elevated esterase were confirmed by biochemical assay. There were significant differences in elevated esterase activity between the temephos selected groups and the non-selected group. However no significant difference between the type form and the pale form was found. Besides the elevated esterase, there was no change in monooxygenase activity and no evidence of insensitive acetylcholinesterease for all temephos selected groups. These results suggest that temephos resistance could be developed in Ae. aegypti under selection pressure and that the main mechanism is based only on esterase detoxification.en_US
dc.description.affiliationInsecticide Research Unit, Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. pungasem@hotmail.comen_US
dc.identifier.citationPaeporn P, Komalamisra N, Deesin V, Rongsriyam Y, Eshita Y, Thongrungkiat S. Temephos resistance in two forms of Aedes aegypti and its significance for the resistance mechanism. The Southeast Asian Journal of Tropical Medicine and Public Health. 2003 Dec; 34(4): 786-92en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34323
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2003_34_4/16-3099.pdfen_US
dc.subject.meshAedes --drug effectsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDengue Hemorrhagic Fever --prevention & controlen_US
dc.subject.meshInsect Vectors --drug effectsen_US
dc.subject.meshInsecticide Resistance --geneticsen_US
dc.subject.meshInsecticides --pharmacologyen_US
dc.subject.meshLarva --drug effectsen_US
dc.subject.meshSelection (Genetics)en_US
dc.subject.meshTemefos --pharmacologyen_US
dc.titleTemephos resistance in two forms of Aedes aegypti and its significance for the resistance mechanism.en_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
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