Serodiagnosis of smear and culture-negative neurotuberculosis with enzyme linked immunosorbent assay for anti A-60 immunoglobulins.

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dc.contributor.authorGhoshal, Ujjalaen_US
dc.contributor.authorKishore, Janaken_US
dc.contributor.authorKumar, Birendraen_US
dc.contributor.authorAyyagari, Archanaen_US
dc.date.accessioned2003-07-18en_US
dc.date.accessioned2009-05-29T12:25:03Z
dc.date.available2003-07-18en_US
dc.date.available2009-05-29T12:25:03Z
dc.date.issued2003-07-18en_US
dc.description.abstractEarly diagnosis of neurotuberculosis (NTB), useful in prevention of mortality and morbidity, remains a challenge despite availability of several tests. An ELISA test to detect IgG and IgM antibodies against Mycobacterial antigen A-60 (Anda Biologicals, France) was done in 677 specimens; group 1 (NTB): 373 sera and 167 cerebrospinal fluid (CSF), group 2: 100 sera from healthy subjects, group 3: 17 CSF from patients undergoing neurosurgical operations for non-tubercular diseases. Anti-A 60 IgA estimation was done in 99 sera from group 1 and all 100 from group 2. Working dilutions were 1:200 for serum and 1:10 for CSF. Serum IgM and IgG anti-A 60 antibodies were more often detected in group 1 than in 2 (50% Vs 10%, p<.001). Anti-IgG and IgM antibody were detected more often in group 1 than in group 3 (33% Vs 6%, p<.001). In serum and CSF both IgM positivity was more than IgG in 2 subgroups of NTB and these are tubercular meningitis, spinal tuberculosis whereas in tuberculoma IgG positivity was more as compared to other 2 groups. Sera were more often positive than CSF (50% Vs 33%, p<.001). Of 32 patients, in whom magnetic resonance imaging (MRI) was done, 15/18 (83%) patients with suggestive findings in MRI had a positive ELISA (IgG or IgM). AntiA-60 antibody is a useful aid in the diagnosis of NTB, especially in smear and culture negative NTB where one does not have much diagnostic opportunities to choose from.en_US
dc.description.affiliationDepartment of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.en_US
dc.identifier.citationGhoshal U, Kishore J, Kumar B, Ayyagari A. Serodiagnosis of smear and culture-negative neurotuberculosis with enzyme linked immunosorbent assay for anti A-60 immunoglobulins. Indian Journal of Pathology & Microbiology. 2003 Jul; 46(3): 530-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/74567
dc.language.isoengen_US
dc.source.urihttps://www.ijpmonline.orgen_US
dc.subject.meshAntibodies, Bacterial --blooden_US
dc.subject.meshAntigens, Bacterialen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHumansen_US
dc.subject.meshMycobacterium tuberculosis --immunologyen_US
dc.subject.meshSerologic Testsen_US
dc.subject.meshTuberculosis, Central Nervous System --diagnosisen_US
dc.titleSerodiagnosis of smear and culture-negative neurotuberculosis with enzyme linked immunosorbent assay for anti A-60 immunoglobulins.en_US
dc.typeJournal Articleen_US
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