Detection of Plasmodia in acridine orange stained capillary tubes (the QBC system).

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dc.contributor.authorPornsilapatip, Jen_US
dc.contributor.authorNamsiripongpun, Ven_US
dc.contributor.authorWilde, Hen_US
dc.contributor.authorHanvanich, Men_US
dc.contributor.authorChutivongse, Sen_US
dc.date.accessioned2009-05-27T16:30:29Z
dc.date.available2009-05-27T16:30:29Z
dc.date.issued1990-12-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe sensitivity, specificity and convenience of carrying out malaria diagnosis in acridine orange stained capillary tubes using a fluorescent microscope (the QBC system) was compared to screening for Plasmodia on conventional Giemsa stained thick smears. A dilution study revealed that the QBC is able to detect Plasmodia in as low a dilution as 5 organisms per ul. The QBC system was evaluated at a district hospital in Thailand. A preliminary study of 186 patients compared the QBC system to the routine malaria screening procedure (screening up to 30 microscopic fields on a thick smear). The sensitivity of the QBC was found to be 98.9% with a specificity of 94.4%. A second combined series of 465 febrile subjects were screened by thick smear and these results were compared to the QBC. 202 were positive for malaria on both QBC and thick smear. Sensitivity in this study was found to be 99.5% (202/203) and the specificity was 94.6% (248/262). When both series were combined, there were 14 QBC malaria positives that were not detected on thick smear, and 2 QBC malaria false negatives among the 651 patients studied. The parasite densities in these cases were between 10 and 320,000 organisms/microliters. The QBC system provided only a crude estimate of the level of parasitemia. The species of Plasmodia (P. falciparum and P. vivax) were correctly identified on QBC in 78% of cases.en_US
dc.description.affiliationQueen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok.en_US
dc.identifier.citationPornsilapatip J, Namsiripongpun V, Wilde H, Hanvanich M, Chutivongse S. Detection of Plasmodia in acridine orange stained capillary tubes (the QBC system). The Southeast Asian Journal of Tropical Medicine and Public Health. 1990 Dec; 21(4): 534-40en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35369
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAcridine Orange --diagnostic useen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAzure Stains --diagnostic useen_US
dc.subject.meshEvaluation Studies as Topicen_US
dc.subject.meshHumansen_US
dc.subject.meshMalaria --epidemiologyen_US
dc.subject.meshMass Screening --methodsen_US
dc.subject.meshMicroscopy, Fluorescence --methodsen_US
dc.subject.meshPlasmodium falciparumen_US
dc.subject.meshPlasmodium vivaxen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshThailand --epidemiologyen_US
dc.titleDetection of Plasmodia in acridine orange stained capillary tubes (the QBC system).en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
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