Acid and Alkaline Hydrolysis Studies of Stevioside and Rebaudioside A.
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Date
2011-10
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Abstract
Hydrolysis studies of the two diterpene glycosides stevioside and rebaudioside A that
were isolated from Stevia rebaudiana were performed using acid and alkaline conditions. Acid
hydrolysis was carried out using H2SO4 whereas alkaline hydrolysis was performed using NaOH.
Using these methods, partial hydrolyzed glycosides of stevioside and rebaudioside A and their
sugar residues were identified from the alkaline and acid hydrolysis studies respectively. The
structures of the acid and alkaline hydrolysis products were achieved on the basis of extensive
NMR and high resolution mass spectral data, and in comparison with the data reported in the
literature as well as TLC comparison with their corresponding standard compounds.
Description
Keywords
Stevioside, Rebaudioside A, Diterpene glycosides, Acid and Alkaline hydrolysis, Spectral data, TLC comparison
Citation
Chaturvedula Venkata Sai Prakash, Prakash Indra. Acid and Alkaline Hydrolysis Studies of Stevioside and Rebaudioside A. Journal of Applied Pharmaceutical Science. 2011 Oct; 1(8): 104-108.