Pongamia pinnata seed cake: A promising and inexpensive substrate for production of protease and lipase from Bacillus pumilus SG2 on solid-state fermentation.
Loading...
Date
2011-12
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The production of a protease and a lipase from Bacillus pumilus SG2 on solid-state fermentation using Pongamia pinnata seed cake as substrate was studied. The seed cake was proved to be a promising substrate for the bacterial growth and the enzyme production. The initial pH, incubation time and moisture content were optimized to achieve maximal enzyme production. Maximum protease production was observed at 72 h and that of the lipase at 96 h of incubation. The production of protease (9840 U/g DM) and lipase (1974 U/g DM) were maximum at pH 7.0 and at 60% moisture content. Triton X-100 (1%) was proved to be an effective extractant for the enzymes and their optimal activity was observed at alkaline pH and at 60ÂșC. The molecular mass of the protease and lipase was 24 and 40 kDa, respectively. Both the enzymes were found to be stable detergent additives. The study demonstrated that inexpensive and easily available Pongamia seed cake could be used for production of industrially important enzymes, such as protease and lipase.
Description
Keywords
Lipase, Protease, Solid state fermentation, Pongamia pinnata seed cake
Citation
Sangeetha R, Geetha A, Arulpandi I. Pongamia pinnata seed cake: A promising and inexpensive substrate for production of protease and lipase from Bacillus pumilus SG2 on solid-state fermentation. Indian Journal of Biochemistry & Biophysics. 2011 Dec; 48(6): 435-439.