Human growth hormone gene expression in vitro following rAAV2/1-mediated gene transfer.
| dc.contributor.author | Qin, Ying | |
| dc.contributor.author | Tian, Ya-ping | |
| dc.date.accessioned | 2011-10-18T05:26:41Z | |
| dc.date.available | 2011-10-18T05:26:41Z | |
| dc.date.issued | 2011-06 | |
| dc.description.abstract | Background: Viral vectors can produce longer-lasting effects than a recombinant protein. Objective: Develop a system of sustained GH1 (a human growth hormone gene) transfer and expression in baby hamster kidney cell (BHK-21) line using recombinant adeno-associated viral vectors pseudotyped with viral capsids from serotype 1 (rAAV2/1). Methods: The expression of GH1 in vitro was examined by reverse transcription polymerase chain reaction and Western blot analysis. Effects of GH1 on cell proliferation were measured by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT) experiments. Mice serum IGF-1 and blood glucose, after injection of virus infected BHK-21 cells, were measured by enzyme-linked immunosorbent assays. Results: rAAV2/1-mediated GH1 gene transfer occurred effectively in vitro after 48 hours of transduction at 1 105 vg/mL (multiplicities of infection). MTT experiments indicated notable effects of the GH1 gene on cell proliferation in vitro. Subsequent animal experiment suggested that the injection of virus infected BHK-21 cells might induce increase of serum IGF-1. Conclusion: Our study shows the feasibility of rAAV2/1-mediated GH gene delivery in vitro, applicable for future experimental and clinical investigations. | en_US |
| dc.identifier.citation | Asian Biomedicine (Research Reviews and News); Vol. 5 No. 3 June 2011; 381-387. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/135007 | |
| dc.language.iso | en | en_US |
| dc.publisher | Asian Biomedicine (Research Reviews and News) | en_US |
| dc.rights | Asian Biomedicine (Research Reviews and News) | en_US |
| dc.source.uri | https://abm.digitaljournals.org/index.php/abm/article/viewFile/671/449 | en_US |
| dc.subject | Baby hamster kidney cell 21 | en_US |
| dc.subject | growth hormone | en_US |
| dc.subject | insulin-like growth factor 1, | en_US |
| dc.subject | recombinant adenoassociated virus vector | en_US |
| dc.title | Human growth hormone gene expression in vitro following rAAV2/1-mediated gene transfer. | en_US |
| dc.type | Article | en_US |