Molecular screening of virulence genes in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolated from clinical specimens in Northwest Iran.

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dc.contributor.authorHasani, A
dc.contributor.authorSharifi, Y
dc.contributor.authorGhotaslou, R
dc.contributor.authorNaghili, B
dc.contributor.authorHasani, A
dc.contributor.authorAghazadeh, M
dc.contributor.authorMilani, M
dc.contributor.authorBazmani, A
dc.date.accessioned2012-12-18T07:29:23Z
dc.date.available2012-12-18T07:29:23Z
dc.date.issued2012-04
dc.description.abstractPurpose: The present study screened clinical isolates of Enterococcus faecalis and Enterococcus faecium to determine the prevalence of high-level gentamicin-resistant enterococci and the potential virulence genes among them. Materials and Methods: Clinical enterococcal isolates were obtained from three university teaching hospitals in Northwest Iran. Isolated enterococci were identified phenotypically followed by antibiotic susceptibility testing. Multiplex PCR was performed for the detection of genus, species-specific targets, gentamicin resistance, and potential virulence genes. Results: Of 220 enterococcal isolates, 133 (60.45%) isolates were identified as high-level gentamicin-resistant. Of these isolates, 79 (59.4%) and 54 (40.6%) were E. faecalis and E. faecium, respectively. All high-level gentamicin-resistant strains carried aac(6′)Ie-aph(2″)Ia. Of 220 isolates, 65.9% were positive for gelE, and 55%, 53.6%, 51.8%, and 49.5% of isolates were positive for cpd, asa1, ace, and esp, respectively. Phenotypically detected β-haemolytic strains (19.54%) were found to possess cylL ls MAB. Conclusion: The study revealed that high-level gentamicin-resistance was related to the presence of aac(6′)Ie-aph(2″)Ia. Isolated enterococci harboured potential virulence determinants, which were more common among E. faecalis than among E. faecium strains.en_US
dc.identifier.citationHasani A, Sharifi Y, Ghotaslou R, Naghili B, Hasani A, Aghazadeh M, Milani M, Bazmani A. Molecular screening of virulence genes in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolated from clinical specimens in Northwest Iran. Indian Journal of Medical Microbiology. 2012 Apr-June; 30(2): 175-181.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143941
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2012;volume=30;issue=2;spage=175;epage=181;aulast=Hasanien_US
dc.subjectEnterococcien_US
dc.subjectgentamicin resistanceen_US
dc.subjectMultiplex PCRen_US
dc.subjectvirulence genesen_US
dc.titleMolecular screening of virulence genes in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolated from clinical specimens in Northwest Iran.en_US
dc.typeArticleen_US
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