Characterization of plasmid mediated AmpC producing Escherichia coli clinical isolates from a tertiary care hospital in South India.

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dc.contributor.authorChakraborty, Arindam
dc.contributor.authorAdhikari, Prabha
dc.contributor.authorShenoy, Shalini
dc.contributor.authorSaralaya, Vishwas
dc.date.accessioned2015-02-13T07:08:42Z
dc.date.available2015-02-13T07:08:42Z
dc.date.issued2014-04
dc.description.abstractContext: Plasmid mediated AmpC (pAmpC) β-lactamase producing Escherichia coli are an emerging problem worldwide as they are now exhibiting resistance to multiple classes of antibiotics and are a major cause of therapeutic failure. Aims: The aim of this study was to characterize pAmpC β-lactamase producing extraintestinal E. coli, their phylogenetic distribution, resistance pattern, treatment options, and impact on patient’s clinical outcome. Settings and Design: This descriptive study was carried out in a multi-specialty tertiary care hospital. Materials and Methods: A total of 300 clinically signifi cant, non-repeat isolates were studied. AmpC disk test was used for phenotypic AmpC-β- lactamase detection. Molecular types of pAmpC were determined by a multiplex polymerase chain reaction (PCR). Phylogenetic analysis was performed by triplex PCR methods. Metallo-beta-lactamase (MBL) detection was done by E test. Antibiogram, treatment, and clinical outcome were collected in a structured proforma. Results: Although 95 isolates (32%) were phenotypically positive for AmpC, PCR detected CIT type of AmpC gene in only 37 isolates. Majority of strains were from phylogroup A (85%) and B1 (58%) which are considered as commensal groups. Co-production of ESBL’s was observed in 33 strains and 5 strains were found to be MBL producers. Most widely prescribed antibiotics were 3rd generation cephalosporins (30%), carbapenems (19%) and aminoglycosides (16%). Conclusions: Plasmid mediated AmpC producing isolates were found to exhibit a high degree of drug resistance, and they mainly belonged to commensal strains possibly due to misuse of antibiotics. Proper antibiotic policy is required to limit the spread of pAmpC producers or else it will lead to a therapeutic dead end in the near future.en_US
dc.identifier.citationChakraborty Arindam, Adhikari Prabha, Shenoy Shalini, Saralaya Vishwas. Characterization of plasmid mediated AmpC producing Escherichia coli clinical isolates from a tertiary care hospital in South India. Indian Journal of Pathology & Microbiology. 2014 Apr-Jun 57 (2): 255-258.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/156024
dc.language.isoenen_US
dc.source.urihttps://www.ijpmonline.org/article.asp?issn=0377-4929;year=2014;volume=57;issue=2;spage=255;epage=258;aulast=Chakrabortyen_US
dc.subjectClinical outcomeen_US
dc.subjectmultidrug-resistanceen_US
dc.subjectmultiplex polymerase chain reactionen_US
dc.subjectphylogenetic groupen_US
dc.subjectplasmid mediated AmpC β-lactamasesen_US
dc.titleCharacterization of plasmid mediated AmpC producing Escherichia coli clinical isolates from a tertiary care hospital in South India.en_US
dc.typeArticleen_US
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