Glutaraldehyde cross-linking of lectins to marker enzymes: protection of binding site by specific sugars.

Simple item page
dc.contributor.authorAppukuttan, P Sen_US
dc.contributor.authorChacko, B Ken_US
dc.contributor.authorGeetha, Men_US
dc.contributor.authorAnnamma, K Ien_US
dc.contributor.authorMathai, Jen_US
dc.date.accessioned2000-04-13en_US
dc.date.accessioned2009-05-27T09:54:57Z
dc.date.available2000-04-13en_US
dc.date.available2009-05-27T09:54:57Z
dc.date.issued2000-04-13en_US
dc.description.abstractThe role of bound specific sugars in protecting the sugar binding activity of several galactose binding proteins during their covalent conjugation to horse radish peroxidase by glutaraldehyde-mediated cross-linking was examined by: a) affinity matrix binding of the conjugate, b) enzyme linked lectin assay and c) hemagglutination assay. During conjugation using 1% glutaraldehyde, protection of jack fruit (Artocarpus integrifolia) lectin (jacalin) activity depended on concentration of specific sugar present during conjugation; optimum protection was offered by 50 mM galactose. This indicated the presence of one or more primary groups at the binding site of jacalin, which is (are) essential for sugar binding. On the other hand, such essential amino group(s) was not indicated at the sugar binding site of the peanut lectin, bovine heart galectin or of the human serum anti alpha-galactoside antibody, since exclusion of sugar during their conjugation to HRP did not diminish sugar binding activity. The differential behavior is discussed in the light of reported differences in sugar specificities. Results indicated that sugar mediated blocking of active site may be used in characterization of the latter in lectins.en_US
dc.description.affiliationDivision of Biochemistry, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, India.en_US
dc.identifier.citationAppukuttan PS, Chacko BK, Geetha M, Annamma KI, Mathai J. Glutaraldehyde cross-linking of lectins to marker enzymes: protection of binding site by specific sugars. Indian Journal of Biochemistry & Biophysics. 2000 Apr; 37(2): 77-80en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28939
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCarbohydratesen_US
dc.subject.meshCattleen_US
dc.subject.meshCross-Linking Reagentsen_US
dc.subject.meshGlutaralen_US
dc.subject.meshHorseradish Peroxidaseen_US
dc.subject.meshHumansen_US
dc.subject.meshLectins --chemistryen_US
dc.subject.meshPlant Lectinsen_US
dc.titleGlutaraldehyde cross-linking of lectins to marker enzymes: protection of binding site by specific sugars.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Biochemistry & Biophysics