The combination of fraction A3 of Cyrtostachys renda Blume with doxorubicin improved cytotoxicity against T47D cell line through cell cycle arrest and apoptotic induction

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dc.contributor.authorSyamsurizal, Sen_US
dc.contributor.authorUtami, DT.en_US
dc.date.accessioned2024-11-30T11:21:20Z
dc.date.available2024-11-30T11:21:20Z
dc.date.issued2024-09
dc.description.abstractCurrently, natural products of herb medicinal plants are widely reported as a supplement to cancer chemotherapy. The cytotoxic effect of a dichloromethane (DCM) extract of Cyrtostachys renda root and its flow cytometric profile against a human breast cancer cell line (T47D) were investigated in this study. The 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromid assay was used to assess the combined cytotoxic effects of fraction A3 C. renda root and doxorubicin. The combination index (CI) criteria was used to analyze the synergistic effect of fraction A3 and doxorubicin. The cell cycle and apoptosis profiles were completed using flow cytometric labeling with propidium iodide/RNase and Annexin V. Cell morphological changes were observed using an inverted microscope. Single cytotoxicity fraction A3 of DCM extract was effective against T47D cells with an IC 50 value of 43.03 ?g/ml. Fraction A3 and doxorubicin at half of IC 50 combined had a strong synergistic effect on T47D cells with a CI of 0.2. The cell cycle analysis results showed that this combination caused cell cycle arrest in the G1 and S phases. This observation is consistent with the results of single fraction A3 and doxorubicin treatments, which resulted in cell cycle arrests in the G1 and S phases, respectively. The result of single fraction A3 treatment, combined with doxorubicin for 24 hours, can also induce apoptosis against T47D cells. When the control and treatment groups were compared, the test cell shape altered.en_US
dc.identifier.affiliationsDepartment of Pharmacy, Faculty of Medicine and Health Sciences, Universitas Jambi, Jambi, Indonesia|Department of Pharmacy, Faculty of Medicine and Health Sciences, Universitas Jambi, Jambi, Indonesia.en_US
dc.identifier.citationSyamsurizal S, Utami DT.. The combination of fraction A3 of Cyrtostachys renda Blume with doxorubicin improved cytotoxicity against T47D cell line through cell cycle arrest and apoptotic induction. Journal of Applied Pharmaceutical Science. 2024 Sept; 14(9): 270-278en_US
dc.identifier.issn2231-3354
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/237707
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber9en_US
dc.relation.volume14en_US
dc.source.urihttps://doi.org/10.7324/JAPS.2024.173607en_US
dc.subjectCell cycle arresten_US
dc.subjectapoptosisen_US
dc.subjectT47D cell lineen_US
dc.subjectfraction A3en_US
dc.subjectdoxorubicinen_US
dc.subjectcombination chemotherapy.en_US
dc.titleThe combination of fraction A3 of Cyrtostachys renda Blume with doxorubicin improved cytotoxicity against T47D cell line through cell cycle arrest and apoptotic inductionen_US
dc.typeJournal Articleen_US
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