Impact of temperature on heat shock protein expression of Bombyx mori cross-breed and effect on commercial traits.
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Date
2011-01
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Abstract
The present study investigated the effect of increasing temperature stress on the thermotolerance of B. mori crossbreed
PM x CSR2 and tissue specific differential expression of heat shock proteins at IVth and Vth instars. The
larvae reared at 25 ± 1oC and 70 ± 5% relative humidity were treated as control. Larvae were subjected to heat
shock temperatures of 34, 38 and 42oC for 3 hr followed by 3 hr recovery. Expression of Heat shock protein 72
were analyzed by SDS-PAGE and confirmed by western blotting analysis. The impact of heat shock on
commercial traits of cocoons was analyzed by following different strategies in terms of acquired thermotolerance
over control. Resistance to heat shock was increased as larval development proceeds and increased
thermotolerance is achieved with the induction of Heat shock protein 72 in the Vth instar larval haemolymph.
Relative influence of heat shock temperatures on commercial traits corresponding to the generation of heat shock
protein 72 was significantly improved over control. In PM x CSR2, cocoon and shell weight significantly
increased to 9.90 and 11.90% over control respectively.
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Keywords
Commercial traits, Bombyx mori, Haemolymph, Heat shock protein, Temperature stress, Thermotolerance
Citation
Howrelia J Hongray, Patnaik Bharat Bhusan, Selvanayagam M, Rajakumar S. Impact of temperature on heat shock protein expression of Bombyx mori cross-breed and effect on commercial traits. Journal of Environmental Biology. 2011 Jan; 32(1): 99-103.