Detection of antigen and antibody in childhood tuberculous meningitis.

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dc.contributor.authorBera, Swatien_US
dc.contributor.authorShende, Nirajen_US
dc.contributor.authorKumar, Satishen_US
dc.contributor.authorHarinath, B Cen_US
dc.date.accessioned2006-08-29en_US
dc.date.accessioned2009-05-30T16:13:59Z
dc.date.available2006-08-29en_US
dc.date.available2009-05-30T16:13:59Z
dc.date.issued2006-08-29en_US
dc.description.abstractOBJECTIVE: Mycobacterium tuberculosis excretory secretory 31 kDa, a serine protease antigen (M. tb ES-31), prepared from Mycobacterium tuberculosis H37Ra culture medium has been shown to have potential in detecting tuberculosis. Precise diagnosis and management of tuberculous meningitis, in children in particular, is essential to curtail mortality and morbidity. METHODS: In this study, M. tb ES-31 antigen, was used in Indirect ELISA to detect tuberculous IgG antibody, in sera and CSF samples while affinity purified anti ES-31 goat antibody was used in sandwich ELISA for detection of tuberculous antigen. In sixty-five samples each of CSF and sera from cases with neurotuberculosis and control with non-tuberculous diseases were collected from Kasturba Hospital, Sevagram. RESULTS: Among the 20 patients suffering from neurotuberculosis the IgG antibody was detected in 17(85%) of CSF and 16(80%) of sera samples, while antigen was detected in 18 (90%) in CSF and 16 (80%) in sera. Overall specificity of the assay for both IgG antibody and antigen detection in CSF was 96% while in sera it was 94% for IgG antibody and 96% for antigen detection. CONCLUSION: This study showed the usefulness of mycobacterial serine protease antigen and its antibody in detecting neurotuberculosis.en_US
dc.description.affiliationDepartment of Biochemistry & Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, Maharashtra, India.en_US
dc.identifier.citationBera S, Shende N, Kumar S, Harinath BC. Detection of antigen and antibody in childhood tuberculous meningitis. Indian Journal of Pediatrics. 2006 Aug; 73(8): 675-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/84372
dc.language.isoengen_US
dc.source.urihttps://medind.nic.in/icb/icbai.shtmlen_US
dc.subject.meshAntibodies, Bacterial --analysisen_US
dc.subject.meshAntigens, Bacterial --analysisen_US
dc.subject.meshChilden_US
dc.subject.meshHumansen_US
dc.subject.meshMycobacterium tuberculosis --immunologyen_US
dc.subject.meshSerine Endopeptidases --immunologyen_US
dc.subject.meshTuberculosis, Meningeal --blooden_US
dc.titleDetection of antigen and antibody in childhood tuberculous meningitis.en_US
dc.typeJournal Articleen_US
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