Preparation of a neutralizing monoclonal antibody specific to serotype 8 rotavirus strains with a super-short RNA pattern.
dc.contributor.author | Ahmed, M U | en_US |
dc.contributor.author | Taniguchi, K | en_US |
dc.contributor.author | Kobayashi, N | en_US |
dc.contributor.author | Urasawa, T | en_US |
dc.contributor.author | Urasawa, S | en_US |
dc.date.accessioned | 2009-05-27T14:35:10Z | |
dc.date.available | 2009-05-27T14:35:10Z | |
dc.date.issued | 1991-03-01 | en_US |
dc.description | The Southeast Asian Journal of Tropical Medicine and Public Health. | en_US |
dc.description.abstract | Extensive cross-neutralization tests have identified seven serotypes of rotavirus. Recently, it has been reported that several human strains (69M, B37 and B38) with a super-short RNA pattern carry new serotype specificity (serotype 8). By using the strain 69M as an immunizing antigen, we prepared a neutralizing monoclonal antibody (designated 69M-2D). In neutralization tests using 18 human and two animal rotavirus strains with different serotype specificities, the 69M-2D antibody was found to neutralize only serotype 8 strains. Also, in an enzyme-linked immunosorbent assay (ELISA), the antibody reacted exclusively with the serotype 8 strains. Thus, the reactivity pattern of the 69M-2D antibody was serotype 8-specific. This antibody should be useful as another reagent for serotyping rotaviruses by ELISA. | en_US |
dc.description.affiliation | Department of Hygiene, Sapporo Medical College, Japan. | en_US |
dc.identifier.citation | Ahmed MU, Taniguchi K, Kobayashi N, Urasawa T, Urasawa S. Preparation of a neutralizing monoclonal antibody specific to serotype 8 rotavirus strains with a super-short RNA pattern. The Southeast Asian Journal of Tropical Medicine and Public Health. 1991 Mar; 22(1): 41-5 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/30528 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.tm.mahidol.ac.th/seameo/publication.htm | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies, Monoclonal --biosynthesis | en_US |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay --methods | en_US |
dc.subject.mesh | Genome, Viral | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Inbred BALB C | en_US |
dc.subject.mesh | Neutralization Tests --methods | en_US |
dc.subject.mesh | RNA, Double-Stranded --immunology | en_US |
dc.subject.mesh | RNA, Viral --immunology | en_US |
dc.subject.mesh | Rotavirus --classification | en_US |
dc.title | Preparation of a neutralizing monoclonal antibody specific to serotype 8 rotavirus strains with a super-short RNA pattern. | en_US |
dc.type | Journal Article | en_US |
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