Preparation of a neutralizing monoclonal antibody specific to serotype 8 rotavirus strains with a super-short RNA pattern.

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dc.contributor.authorAhmed, M Uen_US
dc.contributor.authorTaniguchi, Ken_US
dc.contributor.authorKobayashi, Nen_US
dc.contributor.authorUrasawa, Ten_US
dc.contributor.authorUrasawa, Sen_US
dc.date.accessioned2009-05-27T14:35:10Z
dc.date.available2009-05-27T14:35:10Z
dc.date.issued1991-03-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractExtensive cross-neutralization tests have identified seven serotypes of rotavirus. Recently, it has been reported that several human strains (69M, B37 and B38) with a super-short RNA pattern carry new serotype specificity (serotype 8). By using the strain 69M as an immunizing antigen, we prepared a neutralizing monoclonal antibody (designated 69M-2D). In neutralization tests using 18 human and two animal rotavirus strains with different serotype specificities, the 69M-2D antibody was found to neutralize only serotype 8 strains. Also, in an enzyme-linked immunosorbent assay (ELISA), the antibody reacted exclusively with the serotype 8 strains. Thus, the reactivity pattern of the 69M-2D antibody was serotype 8-specific. This antibody should be useful as another reagent for serotyping rotaviruses by ELISA.en_US
dc.description.affiliationDepartment of Hygiene, Sapporo Medical College, Japan.en_US
dc.identifier.citationAhmed MU, Taniguchi K, Kobayashi N, Urasawa T, Urasawa S. Preparation of a neutralizing monoclonal antibody specific to serotype 8 rotavirus strains with a super-short RNA pattern. The Southeast Asian Journal of Tropical Medicine and Public Health. 1991 Mar; 22(1): 41-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/30528
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal --biosynthesisen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshGenome, Viralen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshNeutralization Tests --methodsen_US
dc.subject.meshRNA, Double-Stranded --immunologyen_US
dc.subject.meshRNA, Viral --immunologyen_US
dc.subject.meshRotavirus --classificationen_US
dc.titlePreparation of a neutralizing monoclonal antibody specific to serotype 8 rotavirus strains with a super-short RNA pattern.en_US
dc.typeJournal Articleen_US
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