Variant FCGR3 genes: transcription and possible origins.

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dc.contributor.authorTong, Yinen_US
dc.contributor.authorJin, Jieen_US
dc.contributor.authorMarget, Matthiasen_US
dc.contributor.authorHumpe, Andreasen_US
dc.contributor.authorNeppert, Juergenen_US
dc.contributor.authorFlesch, Brigitte Ken_US
dc.date.accessioned2009-05-27T17:10:51Z
dc.date.available2009-05-27T17:10:51Z
dc.date.issued2008-12-26en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractThe Fc receptors for human immunoglobulin G (FcgammaR) IIIb are encoded by genes clustered on the long arm of chromosome 1 (band q21 --> 24) and exhibit allelic polymorphisms. Several rare FCGR3B sequences were identified in both white and black donors. However, the origins of these genomic variants are unknown and their transcription has not yet been investigated. Blood from a donor with known FCGR3 variants was used to extract DNA from peripheral blood CD34+ cells, CD19+ B-cells, neutrophils and buccal cells, after which FCGR3 gene sequencing was performed. Additionally, RNA samples from 5 Caucasian individuals containing known variant FCGR3 genes were reverse-transcribed to cDNA and the FCGR3 genes were sequenced. Our results showed that the frequencies of variant clones were higher in B-cell preparations than in CD34+ hematopoietic progenitor cells from peripheral blood and neutrophils. Very high variant frequencies were found in buccal cell-derived clones. Variant cDNA sequences were identified in three of five individuals with known FCGR3 variants. We conclude that FCGR3 gene variants are differentially transcribed between cell types and tissues, increasing the likelihood of the presence of variant FcgammaRIII receptors on the cell surface. The significance of the high number of variant clones in buccal cells, however, is unclear.en_US
dc.description.affiliationDepartment of Haematology, First Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou 310003, China.en_US
dc.identifier.citationTong Y, Jin J, Marget M, Humpe A, Neppert J, Flesch BK. Variant FCGR3 genes: transcription and possible origins. Asian Pacific Journal of Allergy and Immunology. 2008 Dec; 26(4): 223-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36627
dc.language.isoengen_US
dc.subject.meshAdulten_US
dc.subject.meshB-Lymphocytes --immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Frequency --geneticsen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHematopoietic Stem Cells --immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPolymorphism, Geneticen_US
dc.subject.meshReceptors, IgG --geneticsen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleVariant FCGR3 genes: transcription and possible origins.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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