Study of HIV-1 subtypes in serodiscordant couples attending an integrated counselling and testing centre in Mumbai using heteroduplex mobility analysis and DNA sequencing.

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dc.contributor.authorMehta, P R
dc.contributor.authorNema, S
dc.contributor.authorParanjpe, S
dc.contributor.authorIngole, N
dc.contributor.authorWanjare, S
dc.contributor.authorNataraj, G
dc.date.accessioned2012-12-10T08:05:21Z
dc.date.available2012-12-10T08:05:21Z
dc.date.issued2010-10
dc.description.abstractAims: To determine the prevalent subtypes of HIV-1 in serodiscordant couples. Setting: Integrated Counselling and Testing Centre (ICTC), Department of Microbiology. Study Design: Prospective pilot study. Participants: Thirty HIV-1 serodiscordant couples. Inclusion Criteria: a) Documentation of HIV-1 infection in one partner and seronegative status in the other, current history of continued unprotected sexual activity within the partnership, demonstration that they have been in a partnership for at least 1 year and are not currently on highly active antiretroviral therapy HAART; b) willingness of both partners to provide written informed consent including consent to continued couple counselling for 3 months. Materials and Methods: HIV-1 subtyping was carried out by heteroduplex mobility analysis (HMA) by amplifying env region; and DNA sequencing by amplifying gag region. Results: HIV-1 env gene was amplified successfully in 10/30 samples; gag gene, in 25/30 samples; and both env and gag gene were amplified successfully in 5/30 samples. HIV-1 subtype C was detected from 21 samples; subtype B, from 7; and subtype A, from 2. Sample from 1 positive partner was detected as subtype C by env HMA and subtype B by gag sequencing. Conclusion: HIV-1 subtype C was found to be the predominant subtype of HIV-1 in serodiscordant couples attending our ICTC, followed by HIV-1 subtype B and HIV-1 subtype A, respectively. DNA sequencing was found to be the most reliable method for determining the subtypes of HIV-1.en_US
dc.identifier.citationMehta P R, Nema S, Paranjpe S, Ingole N, Wanjare S, Nataraj G. Study of HIV-1 subtypes in serodiscordant couples attending an integrated counselling and testing centre in Mumbai using heteroduplex mobility analysis and DNA sequencing. Indian Journal of Medical Microbiology. 2010 Oct-Dec; 28(4): 290-294.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143726
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2010;volume=28;issue=4;spage=290;epage=294;aulast=Mehtaen_US
dc.subjectDNA sequencingen_US
dc.subjectHIV-1 subtypesen_US
dc.subjectheteroduplex mobility analysisen_US
dc.subjectserodiscordant couplesen_US
dc.titleStudy of HIV-1 subtypes in serodiscordant couples attending an integrated counselling and testing centre in Mumbai using heteroduplex mobility analysis and DNA sequencing.en_US
dc.typeArticleen_US
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