γδ T cells response to Mycobacterium tuberculosis in pulmonary tuberculosis patients using preponderant complementary determinant region 3 sequence.

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dc.contributor.authorXi, Xueyan
dc.contributor.authorHan, Xiqin
dc.contributor.authorLi, Liang
dc.contributor.authorZhao, Zhendong
dc.date.accessioned2011-12-09T07:28:04Z
dc.date.available2011-12-09T07:28:04Z
dc.date.issued2011-09
dc.description.abstractBackground & objectives: The unique immunological functions of γδ T lymphocytes to contribute immunity against Mycobacterium tuberculosis attracted interest of researchers. However, little is known about the specificity of γδ Τ cell in tuberculosis patients and the lack of exact tuberculosis antigen recognized by γδ T cells limited its application. The analysis of complementary determinant region (CDR)3 sequence characteristic in γδ T cells of tuberculosis patients would contribute to understand the distribution specificity of γδ T cell. In present study, we investigated the diversity of the γ9/δ2 T cell immunorepertoire and analysed the specificity of the expressed CDR3 in pulmonary tuberculosis patients. Methods: The total RNA in peripheral blood mononuclear cell of 50 pulmonary tuberculosis patients and 10 healthy controls was extracted. The polymerase chain reaction was used to specifically amplify the CDR3 region of γ9 and δ2 chain. The PCR products were ligated into the pGEM-T easy vector. The plasmid DNA was sequenced using the ABI3700 and the T7 primer. Results: Our findings showed that predominant CDR3 sequence of δ2 chain in pulmonary tuberculosis patients was CACDTLVSTDKLIFGKG. The sequence specifically exists in almost all pulmonary tuberculosis patients. The conserved hydrophobic acid residue in 97 positions is present in the γδ T cell reactive to M. tuberculosis. The length of δ2 CDR3 in pulmonary tuberculosis patients has no relation with the disease progress. Interpretation & conclusions: Our results suggest that γδ T cells appear to use CDR3 sequence to recognise M. tuberculosis antigen. γδ T cells reactive to M. tuberculosis were diverse and polyclonal.en_US
dc.identifier.citationXi Xueyan, Han Xiqin, Li Liang, Zhao Zhendong. γδ T cells response to Mycobacterium tuberculosis in pulmonary tuberculosis patients using preponderant complementary determinant region 3 sequence. Indian Journal of Medical Research. 2011 Sept; 134(3): 356-361.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135768
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193717/en_US
dc.subjectCDR3 regionen_US
dc.subjectgammadelta T cellsen_US
dc.subjectpredominant sequenceen_US
dc.subjectpulmonary tuberculosisen_US
dc.subject.meshAmino Acid Motifs --genetics
dc.subject.meshComplementarity Determining Regions --metabolism
dc.subject.meshDNA Primers --genetics
dc.subject.meshFemale
dc.subject.meshGenetic Vectors
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMolecular Sequence Data
dc.subject.meshMycobacterium tuberculosis --immunology
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshReceptors, Antigen, T-Cell, gamma-delta --immunology
dc.subject.meshReceptors, Antigen, T-Cell, gamma-delta --metabolism
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshSequence Analysis, DNA
dc.subject.meshT-Lymphocytes --immunology
dc.subject.meshT-Lymphocytes --metabolism
dc.subject.meshTuberculosis, Pulmonary --immunology
dc.titleγδ T cells response to Mycobacterium tuberculosis in pulmonary tuberculosis patients using preponderant complementary determinant region 3 sequence.en_US
dc.typeArticleen_US
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