Determination of hepatitis C virus genotypes by melting-curve analysis of quantitative polymerase chain reaction products.

Simple item page
dc.contributor.authorAlfaresi, M Sen_US
dc.contributor.authorElkoush, A Aen_US
dc.date.accessioned2007-07-29en_US
dc.date.accessioned2009-05-28T09:34:45Z
dc.date.available2007-07-29en_US
dc.date.available2009-05-28T09:34:45Z
dc.date.issued2007-07-29en_US
dc.description.abstractHepatitis C virus (HCV) is the major causative agent of non-A and non-B viral hepatitis. Factors associated with disease progression following HCV infection include the viral genotype, the patient's alcohol consumption and viral load. In this study, the COBAS AMPLICOR HCV MONITOR test, a commercially available quantitative assay for HCV RNA, was used for HCV genotyping analysis. Amplification products obtained from 100 HCV-positive cases were subjected to real-time polymerase chain reaction (PCR) typing using a single pair of fluorescence resonance energy transfer (FRET) probes and melting-curve analysis. Of 100 samples tested, two inhibited the PCR, two samples yielded discrepancies between our results and the reference laboratory results and the remaining samples provided correct typing. The present report suggests that HCV genotypes can be determined rapidly with FRET probes directly from COBAS AMPLICOR MONITOR test PCR products.en_US
dc.description.affiliationDepartment of Pathology and Laboratory Medicine - Microbiology Division, Zayed Military Hospital, Abu Dhabi, UAE. uaenow@emirates.net.aeen_US
dc.identifier.citationAlfaresi MS, Elkoush AA. Determination of hepatitis C virus genotypes by melting-curve analysis of quantitative polymerase chain reaction products. Indian Journal of Medical Microbiology. 2007 Jul; 25(3): 249-52en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/54130
dc.language.isoengen_US
dc.source.urihttps://www.ijmm.orgen_US
dc.subject.meshFluorescence Resonance Energy Transferen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHepacivirus --geneticsen_US
dc.subject.meshHepatitis C --virologyen_US
dc.subject.meshHumansen_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshRNA, Viral --blooden_US
dc.subject.meshTransition Temperatureen_US
dc.subject.meshViral Loaden_US
dc.titleDetermination of hepatitis C virus genotypes by melting-curve analysis of quantitative polymerase chain reaction products.en_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Medical Microbiology