Evaluation of an in-house LipL32 polymerase chain reaction for diagnosis of leptospirosis and its correlation with various serological diagnostic techniques

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dc.contributor.authorJose, LRen_US
dc.contributor.authorMahadeviah, SNen_US
dc.contributor.authorBalamurugan, Ven_US
dc.contributor.authorKini, K Ren_US
dc.date.accessioned2020-04-23T07:36:44Z
dc.date.available2020-04-23T07:36:44Z
dc.date.issued2018-09
dc.description.abstractBackground: Leptospirosis is a zoonotic disease of ubiquitous distribution. During rainy seasons, in spring and summer and also during harvest times, the risk of leptospirosis increases as there are chances of frequent contact with infected rat population which is common in Karnataka as farming is a main source of income to the people here. There is a paucity of data regarding the prevalent serovars from Karnataka. This study was undertaken as an attempt to compare a battery of tools such as immunochromatographic test (ICT), microscopic agglutination test (MAT), immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) and in-house polymerase chain reaction (PCR) to detect leptospirosis. Settings and Design: This study using consecutive sampling technique was conducted in a tertiary care centre, Mysore, Karnataka. Subjects and Methods: Samples from 783 suspected cases of leptospirosis in and around Mysore between April 2013 and April 2016 were processed. Samples from 783 patients suspected of leptospirosis were subjected to ICT, IgM ELISA, MAT and in-house PCR. Statistical Analysis Used: The statistical analysis was carried out using SPSS software version. Results: Among 783 samples tested, only 14 (1.7%) were positive by ICT, 341 (44%) were positive by IgM ELISA, 368 (47%) were positive by MAT and 393 (50.2%) were positive by in-house PCR. Conclusions: Mysore can be considered endemic for leptospirosis. The in-house PCR based on LipL32 gene proved to be useful in the early diagnosis of leptospirosis.en_US
dc.identifier.affiliationsDepartment of Microbiology, JSS Medical College, Mysore, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Microbiology, ICAR-NIVEDI, Bengaluru, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, University of Mysore, Mysore, Karnataka, Indiaen_US
dc.identifier.citationJose LR, Mahadeviah SN, Balamurugan V, Kini K R. Evaluation of an in-house LipL32 polymerase chain reaction for diagnosis of leptospirosis and its correlation with various serological diagnostic techniques. Indian Journal of Medical Microbiology. 2018 Sep; 36(3): 385-390en_US
dc.identifier.issn0255-0857
dc.identifier.issn1998-3646
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/198786
dc.languageenen_US
dc.publisherIndian Association of Medical Microbiologistsen_US
dc.relation.issuenumber3en_US
dc.relation.volume36en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmm.IJMM_17_355en_US
dc.subjectImmunochromatographic testen_US
dc.subjectimmunoglobulin M enzyme-linked immunosorbent assayen_US
dc.subjectleptospirosisen_US
dc.subjectLipL 32 polymerase chain reactionen_US
dc.subjectmicroscopic agglutination testen_US
dc.titleEvaluation of an in-house LipL32 polymerase chain reaction for diagnosis of leptospirosis and its correlation with various serological diagnostic techniquesen_US
dc.typeJournal Articleen_US
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