Assessment of the anti-diabetic effect of Bauhinia variegata gold nano-extract against streptozotocin induced diabetes mellitus in rats
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Date
2020-05
Journal Title
Journal ISSN
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Publisher
Open Science Publishers LLP
Abstract
Diabetes mellitus (DM) is the most common type of the metabolic endocrine disorders. It is categorized as the thirdcause of death after heart and cancer diseases. Due to the undesirable side effects of the synthetic anti-diabetic drugs,the medicinal plants were selected for DM treatment. The recent studies directed to use nanotechnology in medicine toovercome restrictions of the drugs that were represented by weak bioavailability, insolubility in water, low absorptionby the intestine, and inability to reach to the appropriate site of action. The study aimed to investigate the antioxidant,antidiabetic, and hypolipidemic effect of Bauhinia variegata (B. variegata) extract before and after incorporating goldnanoparticles (Au-NPs) against DM induced by streptozotocin (STZ) in rats. The present study conducted on 36 malealbino Western rats divided into six groups {control, B. variegata extract treated group, B. variegata gold nano-extracttreated group, diabetic group [injected by STZ intrapretinoally (i.p.) at a dose of 60 mg/kg], diabetic rats treatedwith B. variegata extract, and B. variegata gold nano-extract groups}. The DM related biochemical functions (liverand kidney functions, glucose, insulin, and lipid profile) were assayed. Moreover, the enzymatic and non-enzymaticantioxidants were assayed in addition to lipid peroxidation products in liver and pancreas. Furthermore, the nativeprotein, lipoprotein patterns, and isoenzymes were electrophoretically studied. It was found that the administrationof both B. variegata extract and nano-extract to diabetic rats ameliorated the deleterious effects that occurred asa result of STZ injection and restored the biochemical functions in addition to levels of hepatic and pancreaticantioxidants to normalcy. These findings were supported by the histopathological examination. It was concluded thatB. variegata nano-extract exhibited more antidiabetic effect through restoring the normal architecture of pancreaticβ-cells in addition to the antioxidant and hypolipidemic effect than extract alone, which indicated that the efficacy ofB. variegata extract was increased after the incorporation of Au-NPs.
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Keywords
Diabetes mellitus, Bauhinea variegata, streptozotocin, nanotechnology
Citation
Abdel-Halim Abeer Hamed, Fyiad Amal Abdel Azeem, Aboulthana Wael Mahmoud, El-Sammad Nermin Mohammed, Youssef Ahmed Mahmoud, Ali Mamdouh Moawad. Assessment of the anti-diabetic effect of Bauhinia variegata gold nano-extract against streptozotocin induced diabetes mellitus in rats. Journal of Applied Pharmaceutical Science. 2020 May; 2020 May: 077-091