Detection of heat stable mycobacterial antigen in cerebrospinal fluid by Dot-Immunobinding assay.

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dc.contributor.authorMathai, Aen_US
dc.contributor.authorRadhakrishnan, V Ven_US
dc.contributor.authorSarada, Cen_US
dc.contributor.authorGeorge, S Men_US
dc.date.accessioned2003-03-17en_US
dc.date.accessioned2009-06-03T11:24:33Z
dc.date.available2003-03-17en_US
dc.date.available2009-06-03T11:24:33Z
dc.date.issued2003-03-17en_US
dc.description.abstractBACKGROUND: Isolation of Mycobacterium tuberculosis in cerebrospinal fluid (CSF) specimen in patients with tuberculous meningitis (TBM) is infrequent and carries low sensitivity. Thus development of an alternative laboratory diagnostic test is essential for the early diagnosis and treatment of TBM. OBJECTIVE: A simple, rapid Dot immunobinding assay (Dot-Iba), for the laboratory diagnosis of TBM is devised. This method minimizes the risk of handling infectious material in the laboratory. METHOD: The Dot-Iba was standardized with heat-inactivated M tuberculosis antigen (PPD). The heat-inactivated CSF from TBM and non-TBM patients was similarly assayed and it can detect antigen upto 1ng/ml in CSF. RESULT: A positive result was obtained in all the five culture positive patients with TBM and in 20/25 probable TBM. A negative result was obtained in 38/40 CSF from disease control group. The overall sensitivity and specificity of Dot-Iba was 83.3% and 95% respectively. CONCLUSION: Dot-Iba can be used as an adjunct for the laboratory diagnosis of TBM, particularly in culture negative TBM patients and also in those clinical situations where no laboratory tests are available to distinguish between TBM and partially treated pyogenic meningitis.en_US
dc.description.affiliationDepartment of Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram-695011, Kerala, India.en_US
dc.identifier.citationMathai A, Radhakrishnan VV, Sarada C, George SM. Detection of heat stable mycobacterial antigen in cerebrospinal fluid by Dot-Immunobinding assay. Neurology India. 2003 Mar; 51(1): 52-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/120870
dc.language.isoengen_US
dc.source.urihttps://neurologyindia.comen_US
dc.subject.meshAntigens, Bacterial --analysisen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblotting --methodsen_US
dc.subject.meshMycobacterium tuberculosis --isolation & purificationen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshTuberculosis, Meningeal --cerebrospinal fluiden_US
dc.titleDetection of heat stable mycobacterial antigen in cerebrospinal fluid by Dot-Immunobinding assay.en_US
dc.typeJournal Articleen_US
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