Hepatic and extra-hepatic glutathione-S-transferase activity in wild pigeons (Columba livia).
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Date
1980-09
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Abstract
Glutathione-S-transferase (EC 2.5.1.18) activity was assayed in hepatic and extrahepatic
tissues of pigeons using l-chloro-2,4-dinitrobenzene and l,2-dichloro-4-nitrobenzene as
substrates. Gluthathione-S-transferase activity towards l-chloro-2,4-dinitrobenzene in pigeon
was in the order: kidney >liver >testes >brain >lung>heart. The enzyme activity with 1-
chloro-2,4-dinitrobenzene as substrate was 40-44 times higher in pigeon liver and kidney than
that observed with l,2-dichloro-4-dinitrobenzene as substrate. Km values of hepatic and renal
glutathione transferase with l-chloro-2,4-dinitrobenzene as substrate were 2.5 and 3 mM respectively.
Double reciprocal plots with varying reduced gluthathione concentrations resulted in biphasic
curves with two Km values (liver 0.31 mM and 4mM; kidney 0.36 mM and 1.3 mM). The enzyme
activity was inhibited by oxidized gluthathione in a dose-dependent pattern. 3-Methylcholanthrene
elicited about 50% induction of hepatic glutathione transferase activity whereas phenobarbital
was ineffective.
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Keywords
Avian glutathione-S-transferase, oxidized glutathione, 3-methylcholanthrene;, phenobarbital, inhibition by oxidized glutathione, glutathione-S-transferase
Citation
Kumar Ashwini, Husain M M, Mukhtar Hasan, Murti C R Krishna. Hepatic and extra-hepatic glutathione-S-transferase activity in wild pigeons (Columba livia). Journal of Biosciences. 1980 Sept; 2(3): 181-189.