A New Approach to Screening for Methionine-Producing Bacteria.

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dc.contributor.authorOzulu, U S
dc.contributor.authorNwanah, O U
dc.contributor.authorEkwealor, C C
dc.contributor.authorDike, S K
dc.contributor.authorNwikpo, C L
dc.contributor.authorEkwealor, I A
dc.date.accessioned2015-08-31T11:27:32Z
dc.date.available2015-08-31T11:27:32Z
dc.date.issued2012-01
dc.description.abstractAim: To find a method of screening for active Methionine-producing organisms. Study Design: Examination of cross-section of soil. Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Nigeria between April 2010 and August 2011. Methodology: Bacterial isolates (200) from soil were screened for Methionine producers on solid agar medium seeded with Methionine auxotroph, Escherichia coli. The agar plates were observed for halo growth of the E. coli which indicates Methionine production by the isolate. Methionine production in submerged medium by the isolates was investigated. Results: A total of 24 bacterial isolates were recovered as Methionine producers. Six of the active isolates used for submerged fermentation accumulated Methionine in a range of 0.46 – 1.40mg/ml. A close relationship was established between the nature of the halo growths of E. coli auxotroph on solid agar and the Methionine yields of the active bacterial isolates in submerged medium. Conclusion: It is a new and fast approach to screening for active Methionine producers.en_US
dc.identifier.citationOzulu U S, Nwanah O U, Ekwealor C C, Dike S K, Nwikpo C L, Ekwealor I A. A New Approach to Screening for Methionine-Producing Bacteria. British Microbiology Research Journal. 2012 Jan; 2(1): 36-39.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/162823
dc.language.isoenen_US
dc.source.urihttps://sciencedomain.org/abstract/421en_US
dc.subjectAgar mediumen_US
dc.subjectbacteriaen_US
dc.subjecthalo growthen_US
dc.subjectmethionine productionen_US
dc.subjectsoilen_US
dc.subjectsubmerged mediumen_US
dc.titleA New Approach to Screening for Methionine-Producing Bacteria.en_US
dc.typeArticleen_US
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