Isolation, identification & characterization of Proteus penneri - a missed rare pathogen.

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dc.contributor.authorKishore, Janak
dc.date.accessioned2012-06-13T04:00:17Z
dc.date.available2012-06-13T04:00:17Z
dc.date.issued2012-03
dc.description.abstractBackground & objectives: Indole negative Proteus species are invariably incorrectly identified as P. mirabilis, missing isolates of Proteus penneri. P. penneri is an invasive pathogen capable of causing major infectious diseases still seldom reported in individual cases. We report here the isolation, differentiation, characterization and typing of P. penneri from patients with different clinical infections. Methods: Urine, pus and body fluids collected from patients in intensive care units, wards and out patients departments of a tertiary health care institute from north India were cultured. A total of 61 indole negative Proteus isolates were subjected to extended biochemical tests to differentiate and identify P. penneri from P. mirabilis including failure to produce ornithine decarboxylase (by 0% strains of P. penneri and 100% strains of P. mirabilis) besides P. penneri being uniformly salicin negative, non-utilizer of citrate but ferments sucrose and maltose. Antibiograms and Dienes phenomenon were performed to characterize and type P. penneri isolates besides screening for β-lactamase production. Results: Eight isolates of P. penneri were identified; four from urine, three from abdominal drain-fluid and one from diabetic foot ulcer. P. penneri was isolated as the sole pathogen in all patients having underlying disease; post-operatively. Swarming was not seen in the first strain on primary isolation and was poor in strain-4. All eight isolates were biochemically homologous but multi-drug resistant (MDR) with resistance to 6-8 drugs (up to 12). β-lactamase production was seen in three of five isolates while Dienes phenomenon found four distinct types and discriminated strains differing in resistance even with a single drug. Interpretation & Conclusions: A few additional biochemical tests identified P. penneri isolates; it infected patients with underlying disease and strains were MDR and heterogenous.en_US
dc.identifier.citationKishore Janak. Isolation, identification & characterization of Proteus penneri - a missed rare pathogen. Indian Journal of Medical Research. 2012 Mar; 135(3): 341-345.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/138998
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361870/en_US
dc.subjectDienesen_US
dc.subjectindoleen_US
dc.subjectMDRen_US
dc.subjectproteusen_US
dc.subjectProteus pennerien_US
dc.subjectswarmingen_US
dc.subject.meshDrug Resistance, Multiple
dc.subject.meshHumans
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshProteus Infections --microbiology
dc.subject.meshProteus Infections --urine
dc.subject.meshProteus penneri --classification
dc.subject.meshProteus penneri --drug effects
dc.subject.meshProteus penneri --isolation & purification
dc.subject.meshbeta-Lactamases --metabolism
dc.titleIsolation, identification & characterization of Proteus penneri - a missed rare pathogen.en_US
dc.typeArticleen_US
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