Characterization of Mycobacterium africanum subtype I among cows in a dairy farm in Bangladesh using spoligotyping.

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dc.contributor.authorRahim, Zeauren_US
dc.contributor.authorMöllers, Mariusen_US
dc.contributor.authorte Koppele-Vije, Arianneen_US
dc.contributor.authorde Beer, Jessicaen_US
dc.contributor.authorZaman, Khadizaen_US
dc.contributor.authorMatin, M Aen_US
dc.contributor.authorKamal, Men_US
dc.contributor.authorRaquib, Rubhanaen_US
dc.contributor.authorvan Soolingen, Dicken_US
dc.contributor.authorBaqi, M Aen_US
dc.contributor.authorHeilmann, Frank G Cen_US
dc.contributor.authorvan der Zanden, Adri G Men_US
dc.date.accessioned2009-05-27T16:23:24Z
dc.date.available2009-05-27T16:23:24Z
dc.date.issued2007-07-22en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractAcid-fast bacilli (AFB) were detected in the autopsy lung tissue homogenate samples of four cows (variety Frisian cross) in a dairy farm in Bangladesh. Histopathological examination of the lung tissue demonstrated prominent granulomas, caseating necrosis and calcification indicative of tuberculosis (TB) infection. Mycobacteria could not be cultured from the tissue homogenate samples by Lowenstein-Jensen based conventional culture method though AFB were evident by Ziehl-Neelsen (ZN) staining of the smears of tissue homogenate and in paraffin embedded tissue slices. Spoligotyping performed on DNA extracts of paraffin embedded lung tissue samples confirmed the AFB as a member of the M. tuberculosis complex (MTBC) with a pattern assigned to M. africanum subtype I. This characterization by spoligotyping was confirmed by subjecting M. africanum subtype I isolates from other parts of the world to an alternative identification method based on DNA polymorphism in the gyrB gene (Hain Life Science, GmbH, Nehren, Germany). Since M. africanum is believed to be a human pathogen, general infection in cattle may be a public health threat. The presence of these bacteria in the animal reservoir most likely originated from a caretaker.en_US
dc.description.affiliationInternational Center for Diarrheal Disease Research, Bangladesh, Dhaka, Bangladesh.en_US
dc.identifier.citationRahim Z, Möllers M, te Koppele-Vije A, de Beer J, Zaman K, Matin MA, Kamal M, Raquib R, van Soolingen D, Baqi MA, Heilmann FG, van der Zanden AG. Characterization of Mycobacterium africanum subtype I among cows in a dairy farm in Bangladesh using spoligotyping. The Southeast Asian Journal of Tropical Medicine and Public Health. 2007 Jul; 38(4): 706-13en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35098
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2007_38_4/17-4043.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBacterial Typing Techniquesen_US
dc.subject.meshBangladeshen_US
dc.subject.meshCattleen_US
dc.subject.meshDNA, Bacterial --analysisen_US
dc.subject.meshGenotypeen_US
dc.subject.meshLung --microbiologyen_US
dc.subject.meshMycobacterium bovis --classificationen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshTuberculosis, Bovine --microbiologyen_US
dc.titleCharacterization of Mycobacterium africanum subtype I among cows in a dairy farm in Bangladesh using spoligotyping.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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