Bottle and biochemical assays on temephos resistance in Aedes aegypti in Thailand.

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dc.contributor.authorSaelim, Visuten_US
dc.contributor.authorBrogdon, William Gen_US
dc.contributor.authorRojanapremsuk, Jirasaken_US
dc.contributor.authorSuvannadabba, Saravudhen_US
dc.contributor.authorPandii, Wongdyanen_US
dc.contributor.authorJones, James Wen_US
dc.contributor.authorSithiprasasna, Ratanaen_US
dc.date.accessioned2009-05-27T15:17:08Z
dc.date.available2009-05-27T15:17:08Z
dc.date.issued2005-03-27en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe bottle bioassay measuring the time-mortality rate is a simplified procedure for detecting insecticide resistance. It can be used with a biochemical microplate assay to identify the mechanism involved. This integrated approach was used to detect temephos resistance in Aedes aegypti from Nonthaburi (lowest use) and Roi Et (highest use). Ae. aegypti BKK1 laboratory strain was used as the susceptible reference strain. The appropriate concentration of insecticide for bottle bioassay was determined empirically for Ae. aegypti BKK1 strain and found to be in the range of 800-1,050 microg/bottle. The time-mortality rate at 800 microg/bottle was 170 +/- 8.66 minutes, significantly different from the time-mortality rates in the 850, 900, 950, and 1,050 microg/bottle (p = 0.008) concentrations, which were 135 +/- 15.00, 140 +/- 8.66, 135 +/- 15.00, and 125 +/- 8.66 minutes, respectively. The cut-off concentration selected for resistance detection was 850 microg/bottle. The time-mortality rate for the Roi Et strain was 382 +/- 26.41 minutes, significantly higher than the Nonthaburi (150 +/- 25.10 minutes) and BKK1 strains (145 +/- 20.49 minutes) (p < 0.001). The temephos resistance ratio (RR100) for the Ae. aegypti Roi Et strain was 2.64-fold higher at lethal time (LT100) than for the reference Ae. aegypti BKK1 strain. The mean optical density (OD) value from the biochemical microplate assay for the non-specific esterase of the Roi Et strain was higher than the mean OD for the non-specific esterase of both the Nonthaburi and BKK1 strains. Insensitive acetylcholinesterase was not found to be responsible for the resistance in the field-collected mosquitos. This study suggests that esterase detoxification is the primary cause of resistance in the Ae. aegypti population from Roi Et. Both the bottle bioassay and the biochemical microplate assay were proven to be promising tools for initial detection and field surveillance for temephos resistance.en_US
dc.description.affiliationPattani Provincial Public Health Office, Pattani, Thailand.en_US
dc.identifier.citationSaelim V, Brogdon WG, Rojanapremsuk J, Suvannadabba S, Pandii W, Jones JW, Sithiprasasna R. Bottle and biochemical assays on temephos resistance in Aedes aegypti in Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health. 2005 Mar; 36(2): 417-25en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32394
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2005_36_2/19-3467.pdfen_US
dc.subject.meshAcetylcholinesterase --analysisen_US
dc.subject.meshAedes --drug effectsen_US
dc.subject.meshAnalysis of Varianceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Assay --methodsen_US
dc.subject.meshDengue --prevention & controlen_US
dc.subject.meshHousingen_US
dc.subject.meshHumansen_US
dc.subject.meshInsect Vectors --drug effectsen_US
dc.subject.meshInsecticide Resistanceen_US
dc.subject.meshInsecticides --pharmacologyen_US
dc.subject.meshLarva --drug effectsen_US
dc.subject.meshMosquito Control --methodsen_US
dc.subject.meshTemefos --pharmacologyen_US
dc.subject.meshThailanden_US
dc.subject.meshTime Factorsen_US
dc.titleBottle and biochemical assays on temephos resistance in Aedes aegypti in Thailand.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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