Antimicrobial activity of partially purified bio-compounds of Brevibacillus laterosporus SA14 in saline with added amylase

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dc.contributor.authorDarunee Chanwiriyagulen_US
dc.contributor.authorBenjamas Bunsongsermen_US
dc.contributor.authorRatanaporn Kimapornen_US
dc.contributor.authorMonthon Lertcanawanichakulen_US
dc.date.accessioned2011-02-20T04:32:55Z
dc.date.available2011-02-20T04:32:55Z
dc.date.created2009-12-24en_US
dc.date.issued2009-12-24en_US
dc.description.abstractTo study antimicrobial activity of biocompounds produced from bacteria which may lead to development ofa model for antimicrobial substances production,Brevibacillus laterosporus SA14, isolated from air sample wasstudies. The partially purified biocompounds (PPBs) were prepared from 600 ml Luria Bertani culture broth of2 dayBrev. laterosporus SA 14 by means of 50 % ammonium sulfate precipitation. The PPbs could inhibitthe growth of Gram-positive bacteria (Staphylococcus aureus, Viridans group), Gram-negative bacteria (Escherichiacoli \& Pseudomonas aeruginosa) and yeast-like fungi (Candida albicans). The antimicrobial activity was decreasedwhen incubated with 1 mg/ml amylase for 5 hours at 37 oC. Moreover, the PPBs could show hemolytic activitywhich was disappeared at a titer higher than 1:8, but still had the antimicrobial activity when tested with agar welldiffusion. Interestingly, PPBs at a titer 1:128 could inhibit the growth of microorganisms in saliva when tested withmicrobroth dilution. It is therefore interesting to conduct further study on the application of this PPBs in productionof alcohol free mouthwash.en_US
dc.identifier.citationJournal of Medical Technology and Physical Therapy; Volume 21 Number 3, September - December 2009; 257-267en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/130855
dc.language.isoen_USen_US
dc.publisherKhon Kaen Universityen_US
dc.rightsKhon Kaen Universityen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/article/view/194en_US
dc.titleAntimicrobial activity of partially purified bio-compounds of Brevibacillus laterosporus SA14 in saline with added amylaseen_US
dc.typeORIGINAL ARTICLEen_US
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