Effect of Mangosteen peel fraction extracts on WT1 gene and WT1 protein expression in leukemic cell lines

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dc.contributor.authorS Semsrien_US
dc.contributor.authorC Ampasavateen_US
dc.contributor.authorS Okonogien_US
dc.contributor.authorM Srikamchumen_US
dc.contributor.authorS Anuchapreedaen_US
dc.date.accessioned2011-02-22T06:20:07Z
dc.date.available2011-02-22T06:20:07Z
dc.date.created2009-12-25en_US
dc.date.issued2009-12-25en_US
dc.description.abstractThis study aimed to investigate the effects of mangosteen peel fractional extracts on the cytotoxicity, Wilms' tumor 1 (WT1) mRNA and WT1 protein levels of four leukemic cell lines (K562, U937, Molt4, and HL60) using MTT assay, reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative RT-PCR (real time RT-PCR) techniques, and Western blot analysis in those four leukemic cell lines. The results showed that ethyl acetate fraction of mangosteen peel extract gave the best cytotoxicity to leukemic cells and also decreased rate of cell proliferation in K562 cells. However, the ethanolic fraction extract showed the best result in Molt4 and U937 cells, and the butanol fraction extract showed the best result in HL60 cells when compared to vehicle control. The WT1 gene expression inhibit in all mangosteen active fractions at non-toxic dose (Inhibitory concentration at 20% growth; IC20) in four cell lines and showed that all the active fractions decreased WT1 mRNA levels in a dose and time dependent manner.en_US
dc.identifier.citationSongklanagarind Medical Journal; Vol. 27 No. 5 Sept-Oct 2009; 389-403en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/132174
dc.language.isoen_USen_US
dc.publisherSongklanagarind Medical Journalen_US
dc.rightsFaculty of Medicine, Prince of Songkla University, Songkla, Thailanden_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/SOMJ/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/SOMJ/article/view/315en_US
dc.titleEffect of Mangosteen peel fraction extracts on WT1 gene and WT1 protein expression in leukemic cell linesen_US
dc.typeOriginal Articleen_US
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