Molecular characterization of metallo β-lactamase producing multidrug resistant Pseudomonas aeruginosa from various clinical samples.
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Date
2014-10
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Abstract
Introduction: Pseudomonas aeruginosa is a potent opportunistic nosocomial
human pathogen among Gram-negative bacteria causing various life-threatening
infections in patients from Intensive Care Units. This bacterium has become
resistant to almost all commonly available antibiotics with limited treatment
options. Multi drug resistant P. aeruginosa (MDRPA) is a major cause of concern
among hospital acquired infections. It uses distinctive resistant mechanisms
virtually to all the available antibiotics such as Metallo β-lactamases (MBL)
production, extended spectrum β-lactamase production (ESBL), up regulation
of effl ux systems related genes and decreased outer membrane permeability.
This study was carried out to fi nd one the predominant resistance mechanisms
among MDRPA and the prevalence of corresponding resistance genes.
Materials and Methods: MDRPA isolates collected from various clinical samples
for a period of 1-year (November 2009-Octo ber 2010) were included to detect the
predominant mechanism of resistance using phenotypic and molecular methods.
Molecular characterization of all these isolates was done by polymerase chain
reaction (PCR) for the presence of blaVIM-2, blaIMP-1, blaOXA-23, and blaNDM-1 genes
with specifi c primers. Results: Among 75 MDRPA isolates 84% (63) were MBL
producers. Molecular characterization studied by PCR showed the presence of
blaVIM-2 gene in 13% of MBL producers. Conclusion: The prevalence of MBLs
has been increasing worldwide, particularly among P. aeruginosa, leading to
severe limitations in the therapeutic options for the management. Thus, proper
resistance screening measures and appropriate antibiotic policy can be strictly
adopted by all the healthcare facility providers to overcome these superbugs.
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Keywords
Pseudomonas aeruginosa, metallo β-lactamase genes, blaVIM-2
Citation
Ramakrishnan Kalaivani, Rajagopalan Saranathan, Nair Shashikala, Kenchappa Prashanth, Chandrakesan Sheela Devi. Molecular characterization of metallo β-lactamase producing multidrug resistant Pseudomonas aeruginosa from various clinical samples. Indian Journal of Pathology & Microbiology. 2014 Oct-Dec 57 (4): 579-582.