A culture medium for cultivation of mycobacteria, probably Mycobacterium leprae from Mycobacterium leprae infected tissues.

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dc.contributor.authorKato, Len_US
dc.date.accessioned1985-10-01en_US
dc.date.accessioned2009-05-28T10:07:35Z
dc.date.available1985-10-01en_US
dc.date.available2009-05-28T10:07:35Z
dc.date.issued1985-10-01en_US
dc.description.abstractMycobacterium leprae suspensions were prepared from infected armadillos. The M. leprae cells were inoculated into culture media containing KH2PO4 4.7. g. Na2HPO4 2 g, sodium thioglycolate 1 g, (NH4)2SO4 2 g, MgSO4 0.1 g, ferric ammonium citrate 0.05 g, and lipoic acid (thioctic acid) 0.1 g in one liter distilled water. The solution was enriched with heat killed, sonicated leprosy derived Mycobacterium X or crude mycobactin extract from M. phlei to contain + 0.2 micrograms mycobactin per 1 ml in the final medium. Twenty ml media was distributed into each of 25 ml screw cap tubes and autoclaved for 30 minutes. Positive growth was obtained from seven out of ten specimens when incubated at 34 degrees C. The cultures developed as a sediment in the liquid media, suggesting preference for microaerophylic conditions. No growth was seen on the surface of the semi-solid agar media containing the same ingredients. Latency period of growth was estimated as 10-16 days and time of division as 6 days. Subcultures were obtained. Cells were long, acid fast, arranged side by side or end to end, with a tendency to form long spiral cords or clumps when sedimented on siliconized slides. Pyridine extraction eliminated acid fastness, but not gram positivity. Cultures did not grow on Dubos, Lowenstein or 7H10 media. They produce the disease in the foot pads of mice characteristic of M. leprae. Subcultures remain dependent on the heat killed sonicated mycobacteria, or crude mycobactin extract, and reduced oxygen tension in the media. Results suggest that cultures might be identical to M. leprae.en_US
dc.identifier.citationKato L. A culture medium for cultivation of mycobacteria, probably Mycobacterium leprae from Mycobacterium leprae infected tissues. Indian Journal of Leprosy. 1985 Oct-Dec; 57(4): 728-38en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/54885
dc.language.isoengen_US
dc.subject.meshAmmonium Sulfate --pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArmadillosen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshLeprosy --microbiologyen_US
dc.subject.meshMagnesium Sulfate --pharmacologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMycobacterium leprae --growth & developmenten_US
dc.subject.meshOxazoles --pharmacologyen_US
dc.subject.meshPhosphates --pharmacologyen_US
dc.subject.meshPotassium --pharmacologyen_US
dc.subject.meshPotassium Compoundsen_US
dc.subject.meshThioctic Acid --pharmacologyen_US
dc.subject.meshThioglycolates --pharmacologyen_US
dc.titleA culture medium for cultivation of mycobacteria, probably Mycobacterium leprae from Mycobacterium leprae infected tissues.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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