Circulating levels of tumour necrosis factor-alpha & interferon-gamma in patients with dengue & dengue haemorrhagic fever during an outbreak.

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dc.contributor.authorChakravarti, Anitaen_US
dc.contributor.authorKumaria, Rajnien_US
dc.date.accessioned2006-01-29en_US
dc.date.accessioned2009-05-27T08:03:23Z
dc.date.available2006-01-29en_US
dc.date.available2009-05-27T08:03:23Z
dc.date.issued2006-01-29en_US
dc.description.abstractBACKGROUND & OBJECTIVE: Dengue haemorrhagic fever (DHF) is a severe illness caused by dengue viruses, the pathogenesis of which is not fully understood. Immune enhancement caused by cytokines, interferon and activated complement system is one of the hypotheses proposed to explain the haemorrhagic form of disease. This study was undertaken to investigate the role of pro-inflammatory cytokines tumour necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) in dengue infection, and their possible association with disease severity. METHODS: Blood samples were collected from 60 patients; 28 patients of DF and 32 of DHF (clinically categorized into DF or DHF as per WHO criteria) from September to November 2003 at Delhi. Fifteen healthy individuals were included as control. All the patients were confirmed positive for dengue infection by serology or by reverse transcriptase-polymerase chain reaction (RT-PCR). Serum levels of TNF-alpha and IFN-gamma were determined by ELISA. RESULTS: The levels of both the cytokines were significantly elevated in the disease group as compared to the control group. Significantly higher levels of TNF-alpha; 258.02 pg/ml (P<0.005) were seen in patients having secondary infection, while patients with primary infection had higher level of IFN-gamma; 29.47 pg/ml (P<0.005). TNF-alpha was elevated in the later phase of illness, while IFN-gamma was elevated in early phase also. INTERPRETATION & CONCLUSION: Overproduction of TNF-alpha during secondary infection may have a role in immunopathogenesis of DHF, however, study of other cytokines produced along with IFN-gamma;, during the course of dengue infection is required to know the specific role of IFN-gamma in DI.en_US
dc.description.affiliationDepartment of Microbiology, Maulana Azad Medical College & Associated Lok Nayak Hospital, New Delhi, India. dochak@yahoo.comen_US
dc.identifier.citationChakravarti A, Kumaria R. Circulating levels of tumour necrosis factor-alpha & interferon-gamma in patients with dengue & dengue haemorrhagic fever during an outbreak. Indian Journal of Medical Research. 2006 Jan; 123(1): 25-30en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/23077
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshChilden_US
dc.subject.meshDengue --blooden_US
dc.subject.meshDengue Hemorrhagic Fever --blooden_US
dc.subject.meshHumansen_US
dc.subject.meshIndia --epidemiologyen_US
dc.subject.meshInterferon-gamma --blooden_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshSerologic Testsen_US
dc.subject.meshTumor Necrosis Factor-alpha --blooden_US
dc.titleCirculating levels of tumour necrosis factor-alpha & interferon-gamma in patients with dengue & dengue haemorrhagic fever during an outbreak.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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