Plastocyanin microheterogeneity in Scenedesmus acutus MT8.
dc.contributor.author | Dimitrov, Mitko I | en_US |
dc.contributor.author | Donchev, Anthony A | en_US |
dc.contributor.author | Toromanov, Georgi R | en_US |
dc.contributor.author | Getov, Vladimir I | en_US |
dc.contributor.author | Toncheva-Panova, Tonka G | en_US |
dc.date.accessioned | 2008-10-17 | en_US |
dc.date.accessioned | 2009-05-27T09:59:21Z | |
dc.date.available | 2008-10-17 | en_US |
dc.date.available | 2009-05-27T09:59:21Z | |
dc.date.issued | 2008-10-17 | en_US |
dc.description.abstract | Two total plastocyanin (PC) fractions - loosely bound (lPC) and strongly bound (sPC) were extracted (84% and 16%, respectively) from the homogenate of Scenedesmus acutus MT8. Two-fold isolation-purification procedure including DE-52 chromatography separated IPC into a smaller oxidized [IPC (II)] and a larger reduced [IPC(I)] fractions, in contrast to sPC, where sPC(ll) greatly dominated over sPC(I). Analytical isoelectric focusing (IEF) separated IPC(II) into two main fractions only in the presence of 8 M urea, implying microheterogeneity. Preparative IEF in immobiline pH-gradient of 3.2-4.1 separated IPC(II) into two blue fractions - a more alkaline IPC(II) and a more acidic IPC"(II), which were probably stereoisomers. Their UV-Vis spectra exhibited rarely observed tryptophane (291.5 nm) and some differences at 270 and 287 nm. The exact molecular masses of apo-/holo-lPC (10131 Da/10194 Da) were determined by mass spectrometry. The number of -SH groups was determined from the mass difference between alkylated with 4-vinylpyridine (4-VP) and non-alkylated protein. Additionally, a simple procedure for simultaneous separation of both primary structure and stereoisomers of PC was developed. | en_US |
dc.description.affiliation | Insitute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. mitkod@bio21.bas.bg | en_US |
dc.identifier.citation | Dimitrov MI, Donchev AA, Toromanov GR, Getov VI, Toncheva-Panova TG. Plastocyanin microheterogeneity in Scenedesmus acutus MT8. Indian Journal of Biochemistry & Biophysics. 2008 Oct; 45(5): 310-6 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/29151 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.asp | en_US |
dc.subject.mesh | Chromatography, Ion Exchange | en_US |
dc.subject.mesh | Isoelectric Focusing | en_US |
dc.subject.mesh | Plastocyanin --chemistry | en_US |
dc.subject.mesh | Scenedesmus --chemistry | en_US |
dc.subject.mesh | Spectrometry, Mass, Electrospray Ionization | en_US |
dc.subject.mesh | Spectrophotometry, Ultraviolet | en_US |
dc.title | Plastocyanin microheterogeneity in Scenedesmus acutus MT8. | en_US |
dc.type | Journal Article | en_US |
dc.type | Research Support, Non-U.S. Gov't | en_US |
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