Plastocyanin microheterogeneity in Scenedesmus acutus MT8.

dc.contributor.authorDimitrov, Mitko Ien_US
dc.contributor.authorDonchev, Anthony Aen_US
dc.contributor.authorToromanov, Georgi Ren_US
dc.contributor.authorGetov, Vladimir Ien_US
dc.contributor.authorToncheva-Panova, Tonka Gen_US
dc.date.accessioned2008-10-17en_US
dc.date.accessioned2009-05-27T09:59:21Z
dc.date.available2008-10-17en_US
dc.date.available2009-05-27T09:59:21Z
dc.date.issued2008-10-17en_US
dc.description.abstractTwo total plastocyanin (PC) fractions - loosely bound (lPC) and strongly bound (sPC) were extracted (84% and 16%, respectively) from the homogenate of Scenedesmus acutus MT8. Two-fold isolation-purification procedure including DE-52 chromatography separated IPC into a smaller oxidized [IPC (II)] and a larger reduced [IPC(I)] fractions, in contrast to sPC, where sPC(ll) greatly dominated over sPC(I). Analytical isoelectric focusing (IEF) separated IPC(II) into two main fractions only in the presence of 8 M urea, implying microheterogeneity. Preparative IEF in immobiline pH-gradient of 3.2-4.1 separated IPC(II) into two blue fractions - a more alkaline IPC(II) and a more acidic IPC"(II), which were probably stereoisomers. Their UV-Vis spectra exhibited rarely observed tryptophane (291.5 nm) and some differences at 270 and 287 nm. The exact molecular masses of apo-/holo-lPC (10131 Da/10194 Da) were determined by mass spectrometry. The number of -SH groups was determined from the mass difference between alkylated with 4-vinylpyridine (4-VP) and non-alkylated protein. Additionally, a simple procedure for simultaneous separation of both primary structure and stereoisomers of PC was developed.en_US
dc.description.affiliationInsitute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. mitkod@bio21.bas.bgen_US
dc.identifier.citationDimitrov MI, Donchev AA, Toromanov GR, Getov VI, Toncheva-Panova TG. Plastocyanin microheterogeneity in Scenedesmus acutus MT8. Indian Journal of Biochemistry & Biophysics. 2008 Oct; 45(5): 310-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/29151
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshIsoelectric Focusingen_US
dc.subject.meshPlastocyanin --chemistryen_US
dc.subject.meshScenedesmus --chemistryen_US
dc.subject.meshSpectrometry, Mass, Electrospray Ionizationen_US
dc.subject.meshSpectrophotometry, Ultravioleten_US
dc.titlePlastocyanin microheterogeneity in Scenedesmus acutus MT8.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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