Immunoprophylactic studies on cell wall associated proteins of Mycobacterium tuberculosis H37Ra.
| dc.contributor.author | Dhiman, Neelam | |
| dc.contributor.author | Verma, Indu | |
| dc.contributor.author | Khuller, G K | |
| dc.date.accessioned | 2015-07-31T10:52:23Z | |
| dc.date.available | 2015-07-31T10:52:23Z | |
| dc.date.issued | 1997-01 | |
| dc.description.abstract | The cell wall protein peptidoglycan complex (CW-PPC) of Mycobacterium tuberculosis H37Ra was isolated through sequential extraction of lipids, carbohydrates and soluble proteins. CW-PPC emulsified in FIA was found to induce significant protection in mice against challenge with LD50 dose of M. tuberculosis H37Rv. To identify the immunoprotective components of CW-PPC, the proteins in avid association with peptidogican were dissociated by chemical treatment with trifluoromethanesulthonic acid (CF3CO3H): anisole (2:1). Immunoreactivity of total (CW-Pr) as well as its component proteins i.e., 71, 60 and 45 kDa proteins of cell wall was studied in animals immunized with CW-Pr-FIA. The 71 kDa protein was found to be most immunoreactive giving higher T-cell sensitization and humoral responses. Further, immunization of mice with 71 kDa-FIA demonstrated enhanced T- and B-cell responses. Mice immunized with 71 kDa-FIA gave significantly higher protection (P ≤ 0·05) against intravenous challenge with LD50 dose of M. tuberculosis H37Rv, than BCG immunized animals. The results indicate the potential of 71 kDa cell wall protein as a suitable candidate for Cthe subunit vaccine. | en_US |
| dc.identifier.citation | Dhiman Neelam, Verma Indu, Khuller G K. Immunoprophylactic studies on cell wall associated proteins of Mycobacterium tuberculosis H37Ra. Journal of Biosciences. 1997 Jan; 22(1): 13-21. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/161084 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ias.ac.in/jarch/jbiosci/22/13-21.pdf | en_US |
| dc.subject | Tuberculosis | en_US |
| dc.subject | cell wall; | en_US |
| dc.subject | protein antigens | en_US |
| dc.subject | vaccine | en_US |
| dc.title | Immunoprophylactic studies on cell wall associated proteins of Mycobacterium tuberculosis H37Ra. | en_US |
| dc.type | Article | en_US |