Congenital megacolon: diagnosis using size of sub-mucosal nerve trunk as a criteria.

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dc.contributor.authorSangkhathat, Surasaken_US
dc.contributor.authorTadtayathikom, Kamolnateen_US
dc.contributor.authorPatrapinyokul, Sakdaen_US
dc.contributor.authorMitarnun, Winyouen_US
dc.date.accessioned2009-05-27T20:54:53Z
dc.date.available2009-05-27T20:54:53Z
dc.date.issued2002-02-26en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractCholinergic hyperinnervation in submucosa of aganglionic segment of Hirschsprung's disease has been described. However, objective measurement of the nerve fibers was insufficiently reported. OBJECTIVES: To study the amount and size of hypertrophic submucosal nerve trunks in the aganglionic segment of Hirschsprung's disease, semiquantitatively and evaluate the possibility of using the size as objective diagnostic criteria. MATERIAL AND METHOD: Thirteen specimens from aganglionic segments from Hirschsprung's disease and six specimens from the age-matched control subjected without colonic innervation disorders were studied with the S-100 immunohistochemical technic. The submucosal nerve trunks were counted per ten HPF and measured in width, semiquantitatively. Fiber sizes were stratified into small (<20 micrometers), medium (20-40 micrometers), and large (>40 micrometers). Maximum fiber size in each specimen was also recorded. RESULTS: Average density of submucosal nerve trunk in the aganglionic segment and the control were 12.5 and 3.4 fibers per ten HPF, respectively. The large nerve trunks were detected in 12 of 13 aganglionic specimens and none was detected in the controls. The maximum fiber size in aganglionic segments was 78.2 micrometers, compared to 24.1 micrometers in the control group. The sensitivity and specificity to set the presence of hypertrophic nerve trunk, larger than 40 micrometers as a diagnostic criteria for Hirschsprung's disease were 92.3 and 100 per cent, respectively. The data suggest the use of this size as an objective diagnostic criterion, which may be valuable in rectal suction biopsy specimens.en_US
dc.description.affiliationDepartment of Surgery, Faculty of Medicine, Prince of Songkla University, Thailand.en_US
dc.identifier.citationSangkhathat S, Tadtayathikom K, Patrapinyokul S, Mitarnun W. Congenital megacolon: diagnosis using size of sub-mucosal nerve trunk as a criteria. Journal of the Medical Association of Thailand. 2002 Feb; 85(2): 250-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/43862
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshColon --innervationen_US
dc.subject.meshGanglia --pathologyen_US
dc.subject.meshHirschsprung Disease --diagnosisen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshRectum --innervationen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleCongenital megacolon: diagnosis using size of sub-mucosal nerve trunk as a criteria.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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