Enzyme-linked immunosorbent assay for serodiagnosis of Helicobacter pylori in dyspeptic patients and volunteer blood donors.

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dc.contributor.authorDeankanob, Wutichaien_US
dc.contributor.authorChomvarin, Chariyaen_US
dc.contributor.authorHahnvajanawong, Chariyaen_US
dc.contributor.authorIntapan, Pewpan Men_US
dc.contributor.authorWongwajana, Suwinen_US
dc.contributor.authorMairiang, Pisalnen_US
dc.contributor.authorKularbkaew, Churairaten_US
dc.contributor.authorSangchan, Apichaten_US
dc.date.accessioned2009-05-27T15:09:00Z
dc.date.available2009-05-27T15:09:00Z
dc.date.issued2006-09-06en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractHelicobacter pylori, an important etiological agent in the development of gastritis, peptic ulcer and gastric carcinoma, can be detected by the enzyme-linked immunosorbent assay (ELISA). Our objectives were: (1) to evaluate the efficacy of a commercial ELISA kit (Pyloriset EIA-G III) in sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy for diagnosis of H. pylori infection in Thai dyspeptic patients in Khon Kaen Thailand; and (2) to examine the seroprevalence of H. pylori among blood donors at Srinagarind Hospital's Blood Bank, Khon Kaen University, by the commercial ELISA. Gastric biopsies obtained from 137 dyspeptic patients were diagnosed by culture, rapid urease test (RUT) and histology. Serum samples from the same dyspeptic patients and 100 healthy blood donors were assayed using the commercial ELISA. H. pylori infection in dyspeptic patients was considered positive when the culture or both RUT and histology were positive. Using a cut-off value at a titer of 20 U/ml (as recommended by the manufacturer), we found the commercial ELISA kit had a sensitivity of 93.3%, specificity of 75.3%, PPV of 74.7%, NPV of 93.5% and accuracy of 83.2%. The overall H. pylori seroprevalence in the healthy blood donors was 57%. Of the 100 healthy blood donors, 39 (60.9%) of the males and 18 (50.0%) of the females were seropositive.en_US
dc.description.affiliationDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.en_US
dc.identifier.citationDeankanob W, Chomvarin C, Hahnvajanawong C, Intapan PM, Wongwajana S, Mairiang P, Kularbkaew C, Sangchan A. Enzyme-linked immunosorbent assay for serodiagnosis of Helicobacter pylori in dyspeptic patients and volunteer blood donors. The Southeast Asian Journal of Tropical Medicine and Public Health. 2006 Sep; 37(5): 958-65en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32042
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2006_37_5/18-3810.pdfen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshBlood Donorsen_US
dc.subject.meshDyspepsia --blooden_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshHelicobacter Infections --complicationsen_US
dc.subject.meshHelicobacter pylorien_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSeroepidemiologic Studiesen_US
dc.subject.meshSerologic Testsen_US
dc.subject.meshThailand --epidemiologyen_US
dc.titleEnzyme-linked immunosorbent assay for serodiagnosis of Helicobacter pylori in dyspeptic patients and volunteer blood donors.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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