In vitro development of Haplorchis taichui (Trematoda: Heterophyidae).

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dc.contributor.authorChaithong, Uen_US
dc.contributor.authorSukontason, Ken_US
dc.contributor.authorBoonsriwong, Nen_US
dc.contributor.authorSukontason, K Len_US
dc.contributor.authorPiangjai, Sen_US
dc.date.accessioned2009-05-27T15:34:49Z
dc.date.available2009-05-27T15:34:49Z
dc.date.issued2001-03-04en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractNewly excysted metacercariae of Haplorchis taichui were cultured in a candle jar set at 37 degrees C. Both monophasic culture media [0.85% NaCl, RPMI 1640, RPMI 1640+10% fetal calf serum (FCS)] and diphasic culture media [RPMI 1640 + egg yolk agar, RPMI 1640 + 5%, 10% or 15% blood in blood agar (BA), RMPI 1640 + 5%, 10% and 15% FCS with 5% blood in BA] were used in vitro. Parasites survived for only 1 day in 0.85% NaCl without any development. In RPMI 1640 with egg yolk agar and RMPI 1640 + 5%, 10% FCS, the parasite survived for 3-5 days. In contrast, worms survived for 12-14 days in RPMI 1640 with blood agar without any change in result in a different concentration of blood in BA. The ovary and testes were observed after 3 days incubation in this media. Nevertheless, only 1 parasite in RPMI 1640 with 15% blood in BA had vitellaria and eggs at day 6. RPMI 1640 with blood agar can be used as short-term maintenance for the in vitro culture of H. taichui. However, further studies are needed.en_US
dc.description.affiliationDepartment of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand.en_US
dc.identifier.citationChaithong U, Sukontason K, Boonsriwong N, Sukontason KL, Piangjai S. In vitro development of Haplorchis taichui (Trematoda: Heterophyidae). The Southeast Asian Journal of Tropical Medicine and Public Health. 2001 ; 32 Suppl 2(): 31-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/33139
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCulture Media --analysisen_US
dc.subject.meshCulture Media, Conditioneden_US
dc.subject.meshCyprinidae --parasitologyen_US
dc.subject.meshFish Diseases --parasitologyen_US
dc.subject.meshHeterophyidae --growth & developmenten_US
dc.subject.meshTemperatureen_US
dc.subject.meshTrematode Infections --parasitologyen_US
dc.titleIn vitro development of Haplorchis taichui (Trematoda: Heterophyidae).en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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