Highly conserved nucleotide sequence and its deduced amino acids of the 5'-noncoding region and the capsid protein of a Bangkok isolate dengue-3 virus.

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dc.contributor.authorAttatippaholkun, W Hen_US
dc.contributor.authorAttatippaholkun, M Ken_US
dc.contributor.authorNisalak, Aen_US
dc.contributor.authorVaughn, D Wen_US
dc.contributor.authorInnis, B Len_US
dc.date.accessioned2009-05-27T15:25:46Z
dc.date.available2009-05-27T15:25:46Z
dc.date.issued2000-03-21en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe dengue-3 virus genome encodes an uninterrupted open reading frame (ORF) flanked by 5' and 3' non-coding regions. The order of proteins encoded in dengue-3 virus ORF, as with other flaviviruses, is: Cap 5'-C-prM-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5-3'. The nucleotide sequence of the 5'-noncoding region and the capsid protein of dengue-3virus (a Bangkok isolate: CH53489 isolated by USAMC-AFRIMS in 1973) has been analyzed in both forward and reverse directions. The PCR-based cycle sequencing technique by the enzymatic method of Sanger et al (1977) using a sequencing primer 5'-end labeled with gamma32P-ATP is the method of our choice for sequencing analysis. One cDNA template was prepared by RT-PCR technique starting from the 5'-end nucleotide 1-465 of the dengue-3 genome. In our cycle sequencing experiments, the substitution of 7-deaza-dG was used for dG in DNA eliminated much of the secondary structures that produced gel artifacts. The final sequence result of this cDNA template was established from its sequence data determined on both strands in opposite directions. Alignment between the newly established nucleotide sequence as well as its deduced amino acid sequence of the Bangkok dengue-3 virus and the published sequence data of the dengue-3 prototype (H87) was manipulated by the PC-DOS-GIBIO-DNASIS TM 06-00 (Hitachi Software). According to the deduced amino acid sequence of the Bangkok dengue-3 virus, its C protein was found to be highly positively charged because of large numbers of lysine and arginine. The homology of the nucleotide sequence between the two dengue-3 virus revealed 97%. The deduced amino acid sequences from the nucleotides 95-465 of the two viruses showed the same indicating highly conserved capsid proteins. Multiple alignment of the nucleotide sequences as well as the deduced amino acid sequences among the Bangkok dengue-3 virus and other dengue 3 viruses also confirmed the highly conserved 5'-noncoding regions and the capsid proteins.en_US
dc.description.affiliationDepartment of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationAttatippaholkun WH, Attatippaholkun MK, Nisalak A, Vaughn DW, Innis BL. Highly conserved nucleotide sequence and its deduced amino acids of the 5'-noncoding region and the capsid protein of a Bangkok isolate dengue-3 virus. The Southeast Asian Journal of Tropical Medicine and Public Health. 2000 ; 31 Suppl 1(): 119-25en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32765
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshCapsid --geneticsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDengue Virus --geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshThailanden_US
dc.titleHighly conserved nucleotide sequence and its deduced amino acids of the 5'-noncoding region and the capsid protein of a Bangkok isolate dengue-3 virus.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
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