Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen.
| dc.contributor.author | Yen, C M | en_US |
| dc.contributor.author | Chen, E R | en_US |
| dc.contributor.author | Kojima, S | en_US |
| dc.contributor.author | Kobayashi, M | en_US |
| dc.date.accessioned | 2009-05-27T15:40:32Z | |
| dc.date.available | 2009-05-27T15:40:32Z | |
| dc.date.issued | 1989-03-01 | en_US |
| dc.description | The Southeast Asian Journal of Tropical Medicine and Public Health. | en_US |
| dc.description.abstract | Monoclonal antibodies (MAb) against A cantonensis were produced through fusion of immunised spleen cells from BALB/c mice with NS-1 myeloma cells at a ratio of 10:1. The successful fusion rate on the 3rd day of fusion was 90.1%. Ten MAb were characterised, six of which were IgG1 and the remaining four were IgG2a, IgG2b, IgM and IgA respectively. Among 6 IgG1 MAb, four were A. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile worm antigens. Two other IgG1 MAb showed cross-reaction with other helminthic antigens of Toxocara canis. Ascaris suum. Paragonimus westermani, Dirofilaria immitis, Anisakis Spp, Gnatostoma Spinigerum and Clonorchis sinensis. | en_US |
| dc.description.affiliation | Department of Parasitology, Kaohsiung Medical College, Taiwan, R.O.C. | en_US |
| dc.identifier.citation | Yen CM, Chen ER, Kojima S, Kobayashi M. Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen. The Southeast Asian Journal of Tropical Medicine and Public Health. 1989 Mar; 20(1): 119-24 | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/33379 | |
| dc.language.iso | eng | en_US |
| dc.source.uri | https://www.tm.mahidol.ac.th/seameo/publication.htm | en_US |
| dc.subject.mesh | Angiostrongylus --immunology | en_US |
| dc.subject.mesh | Animals | en_US |
| dc.subject.mesh | Antibodies, Monoclonal --immunology | en_US |
| dc.subject.mesh | Antigens, Helminth --immunology | en_US |
| dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | en_US |
| dc.subject.mesh | Female | en_US |
| dc.subject.mesh | Hybridomas --immunology | en_US |
| dc.subject.mesh | Metastrongyloidea --immunology | en_US |
| dc.subject.mesh | Mice | en_US |
| dc.subject.mesh | Mice, Inbred BALB C | en_US |
| dc.title | Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen. | en_US |
| dc.type | Journal Article | en_US |
| dc.type | Research Support, Non-U.S. Gov't | en_US |
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