Phage therapy of staphylococcal chronic osteomyelitis in experimental animal model.

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dc.contributor.authorKishor, Chandan
dc.contributor.authorMishra, Raghvendra Raman
dc.contributor.authorSaraf, Shyam K
dc.contributor.authorKumar, Mohan
dc.contributor.authorSrivastav, Arvind K
dc.contributor.authorNath, Gopal
dc.date.accessioned2016-08-04T10:33:30Z
dc.date.available2016-08-04T10:33:30Z
dc.date.issued2016-01
dc.description.abstractBackground & objectives: Methicillin resistant Staphylococcus aureus (MRSA) are the commonest cause of osteomyelitis. The aim of this study was to evaluate the role of an alternative therapy i.e. application of S. aureus specific bacteriophages in cases of osteomyelitis caused by MRSA in animal model. Methods: Twenty two rabbits were included in this study. The first two rabbits were used to test the safety of phage cocktail while the remaining 20 rabbits were divided into three groups; group A (n=4) to assess the establishment of osteomyelitis; group B (n=4) osteomyelitis developed but therapy started only after six weeks; and group C (n=12) osteomyelitis developed and therapy started after three weeks. Groups B and C rabbits were treated with four doses of cocktail of seven virulent bacteriophages at the interval of 48 h. Comparison between three groups was made on the basis of observation of clinical, radiological, microbiological, and histopathological examinations. Results: Experimental group rabbits recovered from the illness in the subsequent two weeks of the therapy. Appetite and activity of the rabbits improved, local oedema, erythema and induration subsided. There were minimal changes associated with osteomyelitis in X-ray and histopathology also showed no signs of infection with new bone formation. Control B group rabbits also recovered well from the infection. Interpretation & conclusions: The present study shows a potential of phage therapy to treat difficult infections caused by multidrug resistant bacteria.en_US
dc.identifier.citationKishor Chandan, Mishra Raghvendra Raman, Saraf Shyam K, Kumar Mohan, Srivastav Arvind K, Nath Gopal. Phage therapy of staphylococcal chronic osteomyelitis in experimental animal model. Indian Journal of Medical Research. 2016 Jan; 143(1): 87-94.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/176415
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822375/en_US
dc.subjectMRSAen_US
dc.subjectosteomyelitisen_US
dc.subjectphage therapyen_US
dc.subjectStaphylococcus aureusen_US
dc.titlePhage therapy of staphylococcal chronic osteomyelitis in experimental animal model.en_US
dc.typeArticleen_US
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